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Copyright: Human Genome Center, Inst. Med. Sci., Univ. Tokyo; 1999-2006
Contact: Kenta Nakai |
| Regulated Operon: | cysK |
| Genes | Synonyms | Direction | Genome position | Function | COG ID | Genus clusters |
|---|---|---|---|---|---|---|
| cysK | + | 81768..82694 | cysteine synthetase A | COG0031 | cysK-BAC-1 cysK-BAC-2 cysK-STA cysK-STR |
| Operon evidence: | Northern blotting, RNAse protection assay of the 3' end of the mRNA. |
|---|---|
| Reference: | De Sazieu A, et al. (1997), BSORF |
| Comments: | BSORF shows additional transcripts starting further upstream, but terminating at the same position. The RNAse protection assay showed that transcription terminates at the last T of the T-stretch TTTTTTT. This terminator is slightly leaky. |
| Binding factor |
Regulation | Location | Absolute position | Binding seq.(cis-element) | Experimental evidence |
|---|---|---|---|---|---|
| SigA | Promoter | -41:+8 | 81687..81735 | AAGTGATTGACAAAAAATTTTGAAATTGATAGATTATATACATAATACC |
Van der Ploeg JR, et al. (2001): PE |
| YrzC | Negative | -10:+40 | 81718..81767 | GATTATATACATAATACCAATACAAATAGTCGGAAATTGAGGTGTCGAGA |
Even S, et al. (2006): GS HM AR |
| Terminator sequence | Absolute position | Position from stop codon | Free energy [kcal/mol] |
Downstream of |
|---|---|---|---|---|
| CAAAACTCCCGGTTCGCCGGGAGTTTTTTTATATTTCG >>>>>>> <<<<<<< |
cysK |
|