Regulated Operon: glpD

Genes
Genes Synonyms Direction Genome position Function COG ID Genus clusters
glpD
+ 1004308..1005975 glycerol-3-phosphate dehydrogenase COG0578 glpD-BAC glpD-STA glpK-STA  

Operon evidence: Northern blotting (1.8 kb transcript); S1 nuclease mapping of the 3' end
Reference: Holmberg C, et al. (1990), Beijer L, et al. (1993), Holmberg C & Rutberg L (1991), Holmberg C & Rutberg L (1992)
Comments: The Northern blotting experiment showed a 4.4 kb glpFKD transcript due to readthrough of the glpFK terminator. However, the promoter in front of glpD is the major promoter for glpD transcription. The S1 nuclease mapping results show that transcription ends in the section CCGTTATTTC. Transcriptional terminatation at the stem-loop downstream of the glpD promoter is alleviated by GlpP binding to the ribosomal antiterminator (RAT) sequence in the presence of glycerol-3-phosphate.

Promoters

Binding
factor		 Regulation Location Absolute position Binding seq.(cis-element) Experimental evidence
GlpP Positive +15:+36 1004207..1004228 GGTCCTGAGATGAGGAGAGACC Lindgren V & Rutberg L (1974): DB, enzyme activity
Lindgren V & Rutberg L (1976): DB, glycerol-3-phosphate uptake
Holmberg C & Rutberg B (1991): RG DP DB
Holmberg C & Rutberg B (1992): NB RO 3'-PE
Glatz E, et al. (1996): DB, promoter mutations, phenotype, enzyme activity
Glatz E, et al. (1998): GS
SigA Promoter -46:+18 1004147..1004210 GCAGCTATGGCTTTTAAATAAAGTAATACTATGGTATAATGGTTACAAGTTAATAAGAACGGTC Holmberg C & Rutberg B (1991): PE

Terminator

Terminator sequence Absolute position Position from stop codon Free energy
[kcal/mol]		 Downstream of
GAGGAGAGACCACAGCACCAAAGTGTAAGCATGCACTTTGGCTGTTGTGGTCTCTTTTTCTATTTACCG
     >>>>>>>>>>>>>>>>>>>>>>    <<<<<<<<<<<<<<<<<<<<<<<
1004223..1004271
-1752..-1704
-37.5
 glpD
CATAACGGGCTGTCTGCAGCCCGTTATTTCTTTTTACG
     >>>>>>>    <<<<<<<
1005983..1006000
8..25
-13.5
 glpD

Overview



Upper Region
Copyright: Human Genome Center, Inst. Med. Sci., Univ. Tokyo; 1999-2006
Contact: Kenta Nakai