Regulated Operon: gltX-cysES-yazC-yacOP

Genes
Genes Synonyms Direction Genome position Function COG ID Genus clusters
gltX
+ 111044..112495 glutamyl-tRNA synthetase COG0008 gltX-BAC gltX-STA gltX-STR  
cysE cysA + 112797..113450 serine acetyltransferase COG1045 cysE-BAC-1 cysE-BAC-2 cysE-STA  
cysS snpA + 113447..114847 cysteinyl-tRNA synthetase COG0215  
yazC
+ 114851..115282
COG1939  
yacO
+ 115266..116015
COG0566  
yacP
+ 116022..116534
COG3688  

Operon evidence: Primer extension analysis of cysS; Northern blotting; S1 nuclease mapping
Reference: Gagnon Y, et al. (1994), Pelchat M & Lapointe J (1999), BSORF
Comments: Northern blotting of gltX shows a 1.7 kb transcript. The gltX-cysES-yazC-yacOP transcript is rapidly processed to generate a gltX and a cysES-yazC-yacOP mRNA molecule. RNA cleavage occurs between the last C of the stem-loop and the first T of the T-stretch of the terminator downstream of gltX. Transcriptional readthrough is expected to occur at this stem-loop if uncharged cysteine tRNA binds to the T-box motif in the nascent mRNA transcript. The tRNA anticodon binds to the UGC codon in the sequence AAGUGCGCC in the cysE leader mRNA. Northern blotting results in BSORF show a gltX, a gltX-cysES-yazC-yacOP, and a cysES-yazC-yacOP transcript.

Promoters

Binding
factor		 Regulation Location Absolute position Binding seq.(cis-element) Experimental evidence
SigA Promoter -52:+11 110948..111010 GGCTAACTTGTTTTGATGCCGGGTCTATTTGGTGGTAGAATAGATTCATACATTTTTGCCTGA Gagnon Y, et al. (1994): S1
TrnD-Cys Positive ND 112686..112718 CTTTTCAAACAGAGTGGAACCGCGCGGTTAAAG Gagnon Y, et al. (1994): HM NB OV RG

Terminator

Terminator sequence Absolute position Position from stop codon Free energy
[kcal/mol]		 Downstream of
TTAAAGCGTCTCTGTCATGTTTACATGCAGAGACGCTTTTTTTATTGGGTA
     >>>>>>>>>>>>>>    <<<<<<<<<<<<<
112718..112748
223..253
-23.9
 gltX

Overview



Upper Region
Copyright: Human Genome Center, Inst. Med. Sci., Univ. Tokyo; 1999-2006
Contact: Kenta Nakai