Regulated Operon: lysC

Genes
Genes Synonyms Direction Genome position Function COG ID Genus clusters
lysC ask, aecA - 2908589..2909815 aspartokinase II alpha subunit (aa 1->408) and beta subunit (aa 246->408) COG0527 x1019-STR  

Operon evidence: Genome analysis. Promoter deletions showed that the stemloop in front of lysC may act as a transcriptional antiterminator.
Reference: Chen NY, et al. (1987), Chen NY, et al.: Genetics and biotechnology of Bacilli, vol.2 p.69-74 (1988)
Comments:

Promoters

Binding
factor		 Regulation Location Absolute position Binding seq.(cis-element) Experimental evidence
SigA Promoter -48:+17 2910130..2910194 GCAAAAATAATGTTGTCCTTTTAAATAAGATCTGATAAAATGTGAACTAATTTCATAGTTAGATC Chen NY, et al. (1987): PE
Chen NY, et al. (1988): Genetics and biotechnology of Bacilli, vol.2 p.69-74: PE

Terminator

Terminator sequence Absolute position Position from stop codon Free energy
[kcal/mol]		 Downstream of
AAAAAGGCGGGACTATGCAGTCACCGCCTTTTTGATTACACTG
     >>>>>>>>>    <<<<<<<<<<
2908553..2908575
14..36
-15.7
 lysC
TTAATTGATAAGCAATGAGAGTATTCCTCTCATTGCTTTTTTTATTGTGGA
           >>>>>>>>>>     <<<<<<<<<<
2909885..2909909
-1320..-1296
-16.6
 lysC

Overview



Upper Region
Copyright: Human Genome Center, Inst. Med. Sci., Univ. Tokyo; 1999-2006
Contact: Kenta Nakai