 |
Copyright: Human Genome Center, Inst. Med. Sci., Univ. Tokyo; 1999-2006
Contact: Kenta Nakai |
| Regulated Operon: | nfrA-ywcH |
| Genes | Synonyms | Direction | Genome position | Function | COG ID | Genus clusters |
|---|---|---|---|---|---|---|
| nfrA | ywcG, ipa-43d | - | 3910500..3911249 | FMN-containing NADPH-linked nitro/flavin reductase | COG0778 | |
| ywcH | ipa-44d | - | 3909325..3910326 | COG2141 | x0289-BAC-1 |
| Operon evidence: | Genetic analysis; Northern blotting (2.0 kb transcript); downstream gene is on the opposite strand |
|---|---|
| Reference: | Moch C, et al. (2000) |
| Comments: | The stem-loop between nfrA and ywcH may function as a readthrough terminator, although the Northern blotting experiment indicates a nfrA-ywcH operon. |
| Binding factor |
Regulation | Location | Absolute position | Binding seq.(cis-element) | Experimental evidence |
|---|---|---|---|---|---|
| SigA | Promoter | -41:+4 | 3911265..3911309 | TTTCACTTTTGAGATCACTTTTTTTCGGATATGATAAAAAGTGAT |
Moch C, et al. (2000): PE |
| SigD | Promoter | -41:+5 | 3911264..3911309 | TTTCACTTTTGAGATCACTTTTTTTCGGATATGATAAAAAGTGATA |
Moch C, et al. (1998): PE SDM HM |
| Terminator sequence | Absolute position | Position from stop codon | Free energy [kcal/mol] |
Downstream of |
|---|---|---|---|---|
| AAAAAGCCCTGCCGAATCGGCAGGGCTTTTTCTTATTCAAC >>>>>>>>> <<<<<<<<< |
ywcH | |||
| AAAGCAAACCTCTCCGTCTGGAGGGGTTTTTATGTTTCACGTGTAAC >>>>>>>>>> <<<<<<<<<< |
nfrA |
|