Regulated Operon: nucA-nin

Genes
Genes Synonyms Direction Genome position Function COG ID Genus clusters
nucA comI - 371713..372156 nuclease
nucA-BAC  
nin comJ - 371288..371686

 

Operon evidence: Genome analysis
Reference: Van Sinderen D, et al. (1995), Vosman B, et al. (1988)
Comments: BSORF also shows monocistronic nucA and nin transcripts. The second terminator was found inside the coding region of the downstream gene yckE.

Promoters

Binding
factor		 Regulation Location Absolute position Binding seq.(cis-element) Experimental evidence
ComK Positive ND 372269..372298 CGTATTTTGCTGAAAACAAATATTTCGATC Van Sinderen D, et al. (1995): RG DB GS
ComK Positive ND 372245..372269 CTGCTAAAATTACGATTTCTGCAAT Van Sinderen D, et al. (1995): RG DB GS

Terminator

Terminator sequence Absolute position Position from stop codon Free energy
[kcal/mol]		 Downstream of
AAAACCCCTGAGAGAATAACTCTCAGGGACTCTTTATAAACTTTC
     >>>>>>>>>     <<<<<<<<<
371257..371279
9..31
-18.4
 nin
GTCCAATCCCAGTTGCTCGTTGTCAGATGCGGATTTTTTTTCGTTTTG
      >>>>>>>>>>     <<<<<<<<<<<<
370843..370869
419..445
-7.3
 nin

Overview



Upper Region
Copyright: Human Genome Center, Inst. Med. Sci., Univ. Tokyo; 1999-2006
Contact: Kenta Nakai