Regulated Operon: sacPA-ywdA

Genes
Genes Synonyms Direction Genome position Function COG ID Genus clusters
sacP ipa-49d - 3902669..3904054 phosphotransferase system (PTS) sucrose-specific enzyme IIBC component
scrA-BAC  
sacA ipa-50d - 3901230..3902672 sucrase-6-phosphate hydrolase COG1621  
ywdA ipa-51d - 3900888..3901136

 

Operon evidence: Genome analysis
Reference: Fouet A, et al. (1986), Debarbouille M, et al. (1990), Arnaud M, et al. (1992), Aymerich S & Steinmetz M (1992)
Comments: In-vitro transcription experiments showed that transcription terminates at the stem-loop structure upstream of sacP; transcriptional termination is alleviated in the presence of sucrose by SacT (and to a lesser degree SacY) binding to the ribonucleic antiterminator (RAT) sequence.


Promoters

Binding
factor
Regulation Location Absolute position Binding seq.(cis-element) Experimental evidence
SacT Positive +21:+63 3904140..3904182 ATAAGCGGGATTGTGACTGGTAAAGCAGGCAAGACCTAAAATT Debarbouille M, et al. (1990): DB HM, sucrase activity
Arnaud M, et al. (1992): SDM RG DB
Arnaud M, et al. (1996): GS, in-vitro transcription
SacY Positive +21:+63 3904140..3904182 ATAAGCGGGATTGTGACTGGTAAAGCAGGCAAGACCTAAAATT Arnaud M, et al. (1996): GS, in-vitro transcription
SigA Promoter -38:+13 3904190..3904240 AAAAATAGTTGACGAAAACGCTATCATGATTTATGATGAAAGCGTATTCTT Arnaud M, et al. (1996): RO

Terminator

Terminator sequence Absolute position Position from stop codon Free energy
[kcal/mol]
Downstream of
CAAGACCTAAAATTTGCGTAAATGAAAAAGGATCGCTGTGTCCTTTATTCGTTGGCGAATTTTAGGTCTTTTTT
            >>>>>>>>>>>>>>>>>>>>>      <<<<<<<<<<<<<<<<<<<<
3904095..3904141
-1472..-1426
-18.8
sacP
ACAGGCTGCCGATGGGATCGGCAGTTTTTTCTGTGAAAA
     >>>>>>>>   <<<<<<<<
3900864..3900882
6..24
-13.4
ywdA

Overview



Upper Region






Copyright: Human Genome Center, Inst. Med. Sci., Univ. Tokyo; 1999-2006
Contact: Kenta Nakai