Regulated Operon: speE-speB

Genes
Genes Synonyms Direction Genome position Function COG ID Genus clusters
speE ywhF - 3847809..3848639 spermidine synthase COG0421 speE-BAC  
speB ywhG - 3846876..3847748 agmatinase COG0010  

Operon evidence: Northern blot (1.8kb transcript for speE-speB) The readthrough terminator downstream of speE produces a 0.9kb transcript.
Reference: Presecan E, et al. (1997), Genbank Z80360, Sekowska A, et al. (1998)
Comments:

Promoters

Binding
factor		 Regulation Location Absolute position Binding seq.(cis-element) Experimental evidence
SigA Promoter -53:+12 3848712..3848776 CAGAACAGGACGCATCTTTACTTTTTCCCCGCCATCCTCTATACTTTTTCCTTAGGTGAAAATGA Sekowska A, et al. (1998): PE

Terminator

Terminator sequence Absolute position Position from stop codon Free energy
[kcal/mol]		 Downstream of
GTAAACATCCAGACGATGTCTGGATGTTTTTTTATTCCCAG
     >>>>>>>>>   <<<<<<<<<
3846850..3846870
6..26
-15.1
 speB
GTATGGCGCAGGAGCGAATCTTGCGCTTTTTTACAGGAAAG
     >>>>>>>>     <<<<<<<<
3847778..3847798
11..31
-15.2
 speE

Overview



Upper Region
Copyright: Human Genome Center, Inst. Med. Sci., Univ. Tokyo; 1999-2006
Contact: Kenta Nakai