Regulated operon: tyrS

Regulated Operon:

tyrS

Genes

Genes	Synonyms	Direction	Genome position	Function	COG ID	Genus clusters
tyrS		-	3035645..3036913	tyrosyl-tRNA synthetase	COG0162	tyrS-BAC tyrS-STA tyrS-STR

Operon evidence:	Genome analysis
Reference:	Henkin TM, et al. (1992), Grundy FJ & Henkin TM (1993)
Comments:	Promoter deletions and reporter gene fusions showed that transcription terminates at the stem-loop upstream of tyrS if charged tyrosine tRNA (TrnD-Tyr) binds to the T-box motif in the nascent mRNA transcript. The tRNA anticodon binds to the UAC codon in the sequence GAAUACACU in the tyrS leader mRNA.

Promoters

Binding factor	Regulation	Location	Absolute position	Binding seq.(cis-element)	Experimental evidence
SigA	Promoter	`-43:+18`	3037193..3037253	`AGGCGGCGTTGACACAGGATTTTATTTATGTTAAAAATGATATAGCTTCATATGAAAAGGT`	Henkin TM, et al. (1992): PE
TrnD-Tyr	Positive	ND	3036983..3036999	`AGTAGGGTGGTACCGCG`	Henkin TM, et al. (1992): DP RG Grundy FJ & Henkin TM (1993): RG SDM Grundy FJ, et al. (1994): SDM RG Grundy FJ, et al. (1997): SDM RG Rollins SM, et al. (1997): SDM RG Grundy FJ, et al. (2000): RG SDM NB Gerdeman MS, et al. (2002): GS Grundy FJ, et al. (2002): SDM RG
TrnSL-Tyr1	Positive	ND	3036983..3036999	`AGTAGGGTGGTACCGCG`	Henkin TM, et al. (1992): DP RG Grundy FJ & Henkin TM (1993): RG SDM Grundy FJ, et al. (1994): SDM RG Grundy FJ, et al. (1997): SDM RG Rollins SM, et al. (1997): SDM RG Grundy FJ, et al. (2000): RG SDM NB Gerdeman MS, et al. (2002): GS Grundy FJ, et al. (2002): SDM RG

Terminator

Terminator sequence	Absolute position	Position from stop codon	Free energy [kcal/mol]	Downstream of
`ATAATCAATCGTCCCTTCGTGTAAACGAAGGGGCGTTTTTTATTTTAATT >>>>>>>>>>> <<<<<<<<<<<`	3036948..3036973	-1328..-1303	-22.5	tyrS
`AAAAAGATCCTTTGCCACTGAAGGCAAAGGATCTTTTTGTTTACCGCA >>>>>>>>>>> <<<<<<<<<<<`	3035611..3035638	7..34	-20.7	tyrS

Overview

Upper Region

Copyright: Human Genome Center, Inst. Med. Sci., Univ. Tokyo; 1999-2006
Contact: Kenta Nakai