Regulated Operon: usd-spoIIID-mbl-flhOP

Genes
Genes Synonyms Direction Genome position Function COG ID Genus clusters
usd
- 3747739..3747849

 
spoIIID
- 3747443..3747724 transcriptional regulator
 
mbl
- 3746276..3747277 MreB-like protein
mreB-BAC mreB-LIS  
flhO flgG, yvyA - 3745301..3746113 flagellar basal-body rod protein COG4786  
flhP yvyB - 3744461..3745267 flagellar hook-basal body protein COG4786  

Operon evidence: transcriptional fusions; Northern blotting (1.34 kb usd-spoIIID transcript; 2.8 kb mbl-flhOP transcript)
Reference: Halberg R & Kroos L (1992), Decatur A, et al. (1997), Abhayawardhane Y & Stewart GC (1995), Stevens CM & Errington J (1990), Serizawa M, et al. (2004)
Comments: The usd-spoIIID transcript extends 830 basepairs beyond the spoIIID coding region, which is too short to cover mbl completely. A mbl-specific vegetative promoter may exist inside the spoIIID coding region, leading to the 2.8 kb mbl-flhOP transcript as well as a 1.0 kb monocistronic mbl transcript due to the readthrough terminator downstream of mbl. The Northern blotting results suggest the existence of an internal promoter in front of flhO, leading to a 1.8 kb flhOP transcript.

Promoters

Binding
factor		 Regulation Location Absolute position Binding seq.(cis-element) Experimental evidence
SigE Promoter -38:+5 3747880..3747922 TTTAGCATATTCCCAAAAGAATGCTAATACACTGTTACAAACC Kunkel B, et al. (1989): RG DB
Tatti KM, et al. (1991): PE SDM RG DB OV
Decatur A, et al. (1997): RG

Terminator

Terminator sequence Absolute position Position from stop codon Free energy
[kcal/mol]		 Downstream of
AAAAATTTGTAAAGAACTTATTGTGCTTCCAACTTTTTTTCTATATTT
      >>>>>>>>>>>     <<<<<<<<<<
3747368..3747393
50..75
-3.5
 spoIIID
ACAAACCTCATTCTGAAAAAGAATGAGGTTTTTTTATGAAAAA
     >>>>>>>>>     <<<<<<<<<
3746244..3746266
10..32
-15.1
 mbl
AAAATGGGCGTTTTTCAATCAGCCGCGCATGATAAAAAAAGCGGCTTTTTCGAAATCGTCCTTTTTTTTGTAGTAT
     >>>>>>>>>>>>>>>>>>>>>              <<<<<<<<<<<<<<<<<<<<<
3744396..3744451
10..65
-23.7
 flhP

Overview



Upper Region
Copyright: Human Genome Center, Inst. Med. Sci., Univ. Tokyo; 1999-2006
Contact: Kenta Nakai