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Copyright: Human Genome Center, Inst. Med. Sci., Univ. Tokyo; 1999-2006
Contact: Kenta Nakai |
| Regulated Operon: | valS-folC |
| Genes | Synonyms | Direction | Genome position | Function | COG ID | Genus clusters |
|---|---|---|---|---|---|---|
| valS | - | 2865890..2868532 | valyl-tRNA synthetase | COG0525 | valS-BAC valS-STA | |
| folC | - | 2864538..2865830 | folyl-polyglutamate synthetase | COG0285 | folC-STA |
| Operon evidence: | Northern blotting (4.4 kb transcript) |
|---|---|
| Reference: | Grundy FJ & Henkin TM (1993), Luo D, et al. (1997), Genbank L04520, Genbank X77239 |
| Comments: | The readthrough terminator downstream of valS leads to a 3.0 kb monocistronic valS transcript. Based on homology, transcriptional readthrough is expected to occur at the stem-loop upstream of valS if uncharged valine tRNA binds to the T-box motif in the nascent mRNA transcript. The tRNA anticodon binds to the GUC codon in the sequence AAGGUCGCC in the valS leader mRNA. |
| Binding factor |
Regulation | Location | Absolute position | Binding seq.(cis-element) | Experimental evidence |
|---|---|---|---|---|---|
| SigA | Promoter | -43:+8 | 2868837..2868887 | AGATGATTGACGAATCAAAAACACTTTTACTATAATAAAGAACATAAATAA |
Luo D, et al. (1997): PE NB |
| TrnSL-Val2 | Positive | ND | 2868601..2868643 | GATTGAGTTCATGAAAAAAGGTGGTACCGCGAAAGAGCTTTTC |
Grundy FJ & Henkin TM (1993): HM Luo D, et al. (1997): RG SDM OV |
| Terminator sequence | Absolute position | Position from stop codon | Free energy [kcal/mol] |
Downstream of |
|---|---|---|---|---|
| TACCGCGAAAGAGCTTTTCGTCCTTTTACAGGGATGAAGAGCTCTTTTTTCGTTCTCAG >>>>>>>>>>>>>> <<<<<<<<<<<<<< |
valS | |||
| GGCTCCGGCAGAATCAAAAAAAGATTCTGCCGTTTTTTTCATGTGTA >>>>>>>>>> <<<<<<<<<< |
folC | |||
| GCCGGGGCGGTTTCTAGAGAAACCGCCTTGTCTTATAAAAGA >>>>>>>>> <<<<<<<<< |
valS |
|