Regulated Operon: yaaJ-scr

Genes
Genes Synonyms Direction Genome position Function COG ID Genus clusters
yaaJ
+ 25850..26335
COG0590 x1387-BAC  
scr
+ 26377..26730 small cytoplasmic RNA (scRNA, 4.5S RNA), signal recognition particle-like (SRP) component
 

Operon evidence: S1 mapping analysis of the 3' end
Reference: Struck JC, et al. (1989), Struck JC, et al. (1990)
Comments: Northern blotting experiments listed in BSORF show various measured mRNA transcripts in the yaaJ-scr region. Struck et al. (1990) find a promoter in front of yaaJ, upstream of scr, and suggest that transcription starting at this promoter continues into scr. The S1 nuclease mapping of the 3' end shows that transcription ends in the T-stretch of the indicated terminator structure.


Promoters

Binding
factor
Regulation Location Absolute position Binding seq.(cis-element) Experimental evidence
SigA Promoter ND 25762..25805 CAATCGACAGTCTCCTTTCCGTTTCAGTTATAGTTAATATGTAG Struck JC, et al. (1990): RG HM
SigA Promoter -42:+11 26335..26387 GTACGGTTGCAATTTTTAGGGGAAACAGATATACTTAAGTGTGCATCGTAATA Struck JC, et al. (1989): S1

Terminator

Terminator sequence Absolute position Position from stop codon Free energy
[kcal/mol]
Downstream of
TCAGACTCACCTAATATTAGGTGAGTTTTTTGTTATGTAA
     >>>>>>>>    <<<<<<<<
26707..26726
-23..-4
-12.8
scr

Overview



Upper Region






Copyright: Human Genome Center, Inst. Med. Sci., Univ. Tokyo; 1999-2006
Contact: Kenta Nakai