Regulated Operon: ytmIJKLMNO-ytnIJ-ribR-ytnLM

Genes
Genes Synonyms Direction Genome position Function COG ID Genus clusters
ytmI
- 3007288..3007824
COG0454  
ytmJ tcyJ - 3006465..3007274
COG0834  
ytmK tcyK - 3005639..3006451
COG0834  
ytmL tcyL - 3004898..3005617
COG0765  
ytmM tcyM - 3004161..3004868
COG0765  
ytmN hisP, tcyN - 3003385..3004164
COG1126  
ytmO
- 3002384..3003388
COG2141  
ytnI
- 3002088..3002369
COG0695  
ytnJ
- 3000763..3002091
COG2141  
ribR ytnK - 3000024..3000716 riboflavin kinase COG0196  
ytnL hipO - 2998756..3000006
COG1473  
ytnM
- 2997835..2998737
COG0730  

Operon evidence: Northern blotting (transcript length between 10 kb and 11 kb)
Reference: Solovieva IM, et al. (2004), Solovieva IM, et al. (2005)
Comments:

Promoters

Binding
factor		 Regulation Location Absolute position Binding seq.(cis-element) Experimental evidence
YjbD Negative ND ND ND Erwin KN, et al. (2005): PE RG DB
YtlI Positive -76:-31 3007911..3007956 ATCATTAAAATTACTGAAGTGAAGGTTTACTTTTTTTGATTAGTCA Coppee JY, et al. (2001): DB
Burguiere P, et al. (2005): RG DP DB SDM GS
YrzC Negative -5:+37 3007844..3007885 AAACAATAAAAGCTTAGTAAACAAATAGGAATTATATATTTT Solovieva IM, et al. (2005): promoter mutations, riboflavin synthesis, DB OV, dot-blot analysis
Burguiere P, et al. (2005): RG DB
SigA Promoter -55:+20 3007861..3007935 AAGGTTTACTTTTTTTGATTAGTCAAAGATCAGAGTATGCTTTATAGTATAAACAATAAAAGCTTAGTAAACAAA Burguiere P, et al. (2005): PE
Erwin KN, et al. (2005): PE
Solovieva IM, et al. (2004): NB

Terminator

Terminator sequence Absolute position Position from stop codon Free energy
[kcal/mol]		 Downstream of
TGTAAGAAGCCGGTCGGGCTTCTTTTTTATTTTCCAG
     >>>>>>     <<<<<<
2997744..2997760
75..91
-10.6
 ytnM

Overview



Upper Region
Copyright: Human Genome Center, Inst. Med. Sci., Univ. Tokyo; 1999-2006
Contact: Kenta Nakai