Regulated Operon: yyaF-rpsF-ssb-rpsR

Genes
Genes Synonyms Direction Genome position Function COG ID Genus clusters
yyaF
- 4198867..4199967
COG0012 x0048-BAC  
rpsF
- 4198469..4198756 ribosomal protein S6 (BS9) COG0360 rpsF-STA rpsF-STR  
ssb
- 4197910..4198428 single-strand DNA-binding protein COG0629 ssb-BAC-1 ssb-BAC-2 ssb-OYP ssb-STA ssb-STR x1145-LAB  
rpsR
- 4197627..4197866 ribosomal protein S18
rpsR-BAC rpsR-LAB rpsR-STA rpsR-STR  

Operon evidence: Northern blotting (2.4 kb transcript)
Reference: Lindner C, et al. (2004)
Comments: Internal promoter in front of rpsF, leading to a 1.2 kb transcript. Northern blotting results in BSORF also show a yyaDEF-rpsF-ssb-rpsR transcript.


Promoters

Binding
factor
Regulation Location Absolute position Binding seq.(cis-element) Experimental evidence
ComK Positive ND 4200078..4200094 ATCAAAGGGATTGGCAA Ogura M, et al. (2002): AR RG HM DB
ComK Positive ND 4200061..4200078 AGCTGAAAATGCTTTTTT Ogura M, et al. (2002): AR RG HM DB

Terminator

Terminator sequence Absolute position Position from stop codon Free energy
[kcal/mol]
Downstream of
GTAAAGAGCAAGGACCTTCGGGTTCTTGCTCTTTTTTATAGGGGGG
     >>>>>>>>>>>    <<<<<<<<<<<
4197588..4197613
14..39
-21.4
rpsR

Overview



Upper Region






Copyright: Human Genome Center, Inst. Med. Sci., Univ. Tokyo; 1999-2006
Contact: Kenta Nakai