Regulated Operon: citZ-icd-mdh

Genes
Genes Synonyms Direction Genome position Function COG ID Conserved groups
citZ citA2 - 2981151..2982269
COG0372C citZ-BAC citZ-STA  
icd citC - 2979716..2980987
COG0538C citC-BAC citC-STA citC-BAC citC-STA  
mdh citH - 2978734..2979672
COG0039C mdh-BAC-1 mdh-BAC-2 citM-BAC mdh-BAC-2  

Operon evidence: Genome analysis
Reference: Jin S & Sonenshein AL (1994), Jin S, et al. (1996), Lapidus A, et al. (1997), Genbank AF008220
Comments: internal promoters in front of icd and mdh


Promoters

Binding
factor
Regulation Location Absolute position Binding seq.(cis-element) Experimental evidence
CcpA Negative +80:+97 2982367..2982384 AATGTAAGCATTTTCTTT Kim HJ, et al. (2002): PE DB RG SDM GS
CcpC Negative -11:+5 2982459..2982474 ATAATGAGAATAGGCT Jourlin-Castelli C, et al. (2000): RG GS FT
Kim HJ, et al. (2002): PE DB RG SDM FT
CcpC Negative +21:+44 2982420..2982443 TTATAAAAATTTGTTATGTTCTTT Jourlin-Castelli C, et al. (2000): RG GS FT
Kim HJ, et al. (2002): PE DB RG SDM FT
SigA Promoter -48:+18 2982446..2982511 TATAGGTAAACATTTAACAAATGTCTGATTATTGTTTATAATGAGAATAGGCTTAAACTTAAATAA Jin S, et al. (1994): PE
SigA Promoter -45:+18 2981052..2981114 GCCTCTTGTTCAGACATCAAAATTGGGTTACACTTTAAATTGAATGTTAGGAAAAATCATTTT Jin S, et al. (1994): PE

Terminator

Terminator sequence Absolute position Position from stop codon Free energy
[kcal/mol]
Downstream of
AGAAAGGCTTGCTTAATACAGCCTTTCTCTTTTTACTA
     >>>>          <<<<
2978702..2978719
15..32
-4.8
mdh

Overview



Upper Region






Copyright: Human Genome Center, Inst. Med. Sci., Univ. Tokyo; 1999-2008
Contact: Kenta Nakai