Regulated Operon: gcaD-prs-ctc

Genes
Genes Synonyms Direction Genome position Function COG ID Conserved groups
gcaD tms26 + 56352..57722
COG1207M gcaD-BAC  
prs
+ 57745..58698
COG0462FE prs-BAC prs-STA prs-STR-1 prs-STR-2  
ctc
+ 58783..59397
COG1825J  

Operon evidence: Northern blotting (3.0 kb transcript)
Reference: Horsburgh MJ, et al. (2001), Hilden I, et al. (1995), Moran CP Jr, et al. (1981), Truitt CL, et al. (1988)
Comments: The internal promoter in front of ctc leads to a 0.6 kb monocistronic ctc transcript. Northern blotting results in BSORF show both a gcaD-prs-ctc transcript and longer transcripts.


Promoters

Binding
factor
Regulation Location Absolute position Binding seq.(cis-element) Experimental evidence
SigA Promoter -48:+17 56276..56340 CATGAAGTCTCCTTGAAATCAGAAGATATTTAGGATATATTTTTCTATGGATAAAAGGGATATTG Moran CP Jr, et al. (1982): RO, dinucleotide priming
Henkin TM, et al. (1988): Genetics and biotechnology of Bacilli, vol.2 p.63-67: SDM RG RO
Whipple FW, et al. (1992): RO
SigB Promoter -40:+2 58708..58749 CGAGGTTTAAATCCTTATCGTTATGGGTATTGTTTGTAATAG Ollington JF, et al. (1981): NB DP RO, in-vitro transcription
Moran CP Jr, et al. (1981): FT S1 RO, dinucleotide priming
Tatti KM & Moran CP Jr (1984): RO SDM
Igo MM & Losick R (1986): RG NB DP
Boylan SA, et al. (1993): RG
Voelker U, et al. (1994): 2D PAGE, HB
Petersohn A, et al. (1999): HB

Terminator

Terminator sequence Absolute position Position from stop codon Free energy
[kcal/mol]
Downstream of
CTTAAGGCGTAACCCTCCCGCGGTTACGTCTTTTGTGCTAGAATG
     >>>>>>>>>       <<<<<<<<<
59406..59430
9..33
-13.4
ctc

Overview



Upper Region






Copyright: Human Genome Center, Inst. Med. Sci., Univ. Tokyo; 1999-2008
Contact: Kenta Nakai