Regulated Operon: purEKBCSQLFMNHD

Genes
Genes Synonyms Direction Genome position Function COG ID Conserved groups
purE
+ 698612..699100
COG0041F purE-BAC purE-STA purE-STR x1273-CLO  
purK
+ 699093..700235
COG0026F  
purB
+ 700232..701527
COG0015F purB-STR  
purC
+ 701601..702326
COG0152F purC-BAC purC-STR  
purS yexA + 702319..702573
COG1828F  
purQ
+ 702570..703253
COG0047F  
purL
+ 703237..705465
COG0046F x0046-STR  
purF purB + 705441..706871
COG0034F purB-STR  
purM
+ 706973..708013
COG0150F purM-BAC purM-STR  
purN
+ 708010..708597
COG0299F  
purH purJ + 708594..710132
COG0138F purH-BAC purH-STA purH-STR  
purD
+ 710148..711416
COG0151F purD-BAC purD-STA purD-STR  

Operon evidence: S1 nuclease mapping of 3' end (transcript ends at the T at position 710789); Northern blotting (12.8 kb transcript). About half of the transcripts terminate at the stemloop structure downstream of purF. S1 nuclease and reporter gene experiments showed that guanine can cause transcriptional termination 195 bp downstream of the transcription start site at the antiterminator structure in front of purE. Repression by adenine is mediated by PurR.
Reference: Ebbole DJ & Zalkin H (1987), Ebbole DJ & Zalkin H: Genetics and biotechnology of Bacilli, vol.2 pp.51-55 (1988), Ebbole DJ & Zalkin H (1989), Yoshida K, et al. (2003)
Comments:


Promoters

Binding
factor
Regulation Location Absolute position Binding seq.(cis-element) Experimental evidence
PurR Negative -91:-28 698279..698342 AATTGATCTAAAACACGAACATTAGTAGAATGAATTTTTGTATCGTTCGATAATATCGTTGACA Ebbole DJ & Zalkin H (1987): S1
Ebbole DJ & Zalkin H (1989): RG
Ebbole DJ & Zalkin H (1989): DP RG GS FT
Saxild HH & Nygaard P (1991): Enzyme activity measurement
Weng M, et al. (1995): GS FT DB DP RG
Shin BS, et al. (1997): GS FT SDM
Weng M & Zalkin H (2000): DB RG GS
Saxild HH, et al. (2001): HM RG
Bera AK, et al. (2003): GS DP, ultracentrifugation
Xuan J, et al. (2005): GS SDM RG
Qian J, et al. (2006): Classical genetics
SigA Promoter -45:+20 698325..698389 TCGATAATATCGTTGACATTATCCATGTCCGTTGTTAAGATAAACATGAAATCAAAACACGACCT Ebbole DJ & Zalkin H (1987): S1 PE

Terminator

Terminator sequence Absolute position Position from stop codon Free energy
[kcal/mol]
Downstream of
CGATACATGTACCATGCCCGGTTTGTATTGCTTCCTCATAAGTGCAATGCAGAGCGGGTATTTTTTATTTTCTGA
               >>>>>>>>>>>>>>>>>>>>>    <<<<<<<<<<<<<<<<<<<
698522..698565
-578..-535
-24.5
purE
TGAAAAATGACATAAAGGCAGCGCAGTTCGGCTGCCTTTCTCTTTCTGCCC
                >>>>>>        <<<<<<
706892..706911
21..40
-12.0
purF
GAAAACCCGCAGAATAGCTGCGGGTTTTTTGTTATCAAA
     >>>>>>>     <<<<<<<
711427..711445
11..29
-15.1
purD

Overview



Upper Region






Copyright: Human Genome Center, Inst. Med. Sci., Univ. Tokyo; 1999-2008
Contact: Kenta Nakai