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Copyright: Human Genome Center, Inst. Med. Sci., Univ. Tokyo; 1999-2008
Contact: Kenta Nakai |
| Regulated Operon: | yfhKLM |
| Genes | Synonyms | Direction | Genome position | Function | COG ID | Conserved groups |
|---|---|---|---|---|---|---|
| yfhK | + | 928803..929321 | COG3103T | |||
| yfhL | + | 929406..929738 | COG5658S | |||
| yfhM | + | 929725..930585 | COG0596R |
| Operon evidence: | Northern blotting (1.8 kb transcript) |
|---|---|
| Reference: | Antelmann H, et al. (2000), Huang X, et al. (1999), Genbank D85082 |
| Comments: | Internal promoters in front of yfhL and yfhM, leading to a 1.2 kb and a 0.9 kb transcript, respectively. SubtiList and Genbank D85082 list a terminator after yfhK, but none after yfhM. Northern blotting results in BSORF show a yfhK, a yfhLM, and a yfhKLM transcript. Huang et al. found a sigW-dependent promoter in front of yfhL; the promoter in front of yfhM has not yet been identified. |
| Binding factor |
Regulation | Location | Absolute position | Binding seq.(cis-element) | Experimental evidence |
|---|---|---|---|---|---|
| SigB | Promoter | ND | 928733..928775 | ACATGTTTCACCAGCCTGTCAATCAGGGAATACCACTTATATC |
Antelmann H, et al. (2000): HB |
| SigW | Promoter | -38:+6 | 929352..929395 | ATGCATGAAACATTTCTTCTTTCTGCACGTAACAATGAGAAAGG |
Huang X, et al. (1999): S1 Cao M, et al. (2002): AR |
| Terminator sequence | Absolute position | Position from stop codon | Free energy [kcal/mol] |
Downstream of |
|---|---|---|---|---|
| CATCCGTCTGTCATAATGGCAGACTTTTTCTGTGCGTTT >>>>>>>> <<<<<<<< |
yfhM |
|