Regulated Operon: yfhQ-fabL-sspE

Genes
Genes Synonyms Direction Genome position Function COG ID Conserved groups
yfhQ
+ 935656..936765
COG1194L  
fabL ygaA + 937079..937831
COG1028IQR fabL-BAC  
sspE
+ 937900..938154

 

Operon evidence: Northern blotting (2.6 kb transcript)
Reference: Hackett RH & Setlow P (1987), Yamamoto H, et al. (1999), BSORF
Comments: The gene yfhS is located between yfhQ and fabL on the opposite DNA strand. Internal promoter in front of sspE, leading to a 0.3 kb transcript. The two internal promoters in front of fabL result in a 1.5 kb and a 1.3 kb transcript. The readthrough terminator downstream of yfhQ leads to a 1.2 kb transcript.

Promoters

Binding
factor		 Regulation Location Absolute position Binding seq.(cis-element) Experimental evidence
unknown Promoter -36:+6 935546..935587 CTTTTTCTTTATTACTATTTAATGTAACATATTATCGTACTG Yamamoto H, et al. (1999): NB PE
YfhP Negative ND ND ND Yamamoto H, et al. (1999): PE RG (during exponential growth)
SigA Promoter -43:+5 936596..936643 ACCTGGTATGGAATATTTCAGTGTTTTTCGGTAAAGTAAAACAAGTGT Yamamoto H, et al. (1999): NB PE
SigA Promoter -39:+6 936868..936912 TTGATTGGCCTTCTTCGTTCAAATAAGGCATAATTTGCTGAAAGG Yamamoto H, et al. (1999): NB PE
unknown Promoter -42:+6 936796..936843 CGTGGCTGTAGTCCGCTTCATTGCGCTTCATTCCGCCGCGCGCTTCAA Yamamoto H, et al. (1999): NB PE
RsfA Positive ND ND ND Juan Wu L, et al. (2000): RG DB
SigG Promoter -40:+10 937845..937894 AGAGGAATAGCTATACGATCACCTGCACATTCTAATGACCGTGGAGGTGA Fajardo-Cavazos P, et al. (1991): PE, promoter mutations, RG
Nicholson WL, et al. (1989): PE, nuclease protection, RO
Sun DX, et al. (1989): RO, nuclease protection, SDS-PAGE, RG, RNAP purification, in-vitro transcription
SpoVT Negative ND ND ND Bagyan I, et al. (1996): DB

Terminator

Terminator sequence Absolute position Position from stop codon Free energy
[kcal/mol]		 Downstream of
AAAAAGCACTTCATCTTCGGGTGGAAGTGCTTTTTTCTGTTTGAA
     >>>>>>>>>>    <<<<<<<<<<<
938173..938197
19..43
-14.5
 sspE
TGGCAGGAGAAGCAGCCGCCATCTCGGCTGCTCCGTAAACCATTCTTAAT
     >>>>>>>>>>>>>      <<<<<<<<<<
936733..936761
-32..-4
-18.2
 yfhQ

Overview



Upper Region
Copyright: Human Genome Center, Inst. Med. Sci., Univ. Tokyo; 1999-2008
Contact: Kenta Nakai