Regulated Operon: yjcB-yjcZ-spoVIF

Genes
Genes Synonyms Direction Genome position Function COG ID Conserved groups
yjcB
+ 1252170..1252385

 
yjcZ
+ 1253103..1253252

 
yjcC spoVIF + 1252649..1252951

x1383-BAC  

Operon evidence: Northern blotting (0.5 kb yjcB-yjcZ transcript; 0.3 kb yjcC transcript)
Reference: Kuwana R, et al. (2003)
Comments: Internal promoter upstream of yjcZ produces a 0.2 kb yjcZ transcript, as well as a 0.6 kb yjcZ-yjcC transcript due to readthrough at the terminator downstream of yjcZ. The yjcZ gene was discovered by Kuwana et al.

Promoters

Binding
factor		 Regulation Location Absolute position Binding seq.(cis-element) Experimental evidence
SigK Promoter -45:+12 1252756..1252812 TTTTTGTTTTTTTAACGAGGCGGTCTCGCTCAGCATATATCATAAGCGGGGGGATAA Kuwana R, et al. (2003): NB DB PE
GerE Positive -74:-29 1253007..1253052 TATGATCAATCGTAAATCCGCAAACGCCCTATGCGAATATCCAAGC Kuwana R, et al. (2003): NB DB HM
SigK Promoter -43:+11 1253038..1253091 TGCGAATATCCAAGCCGATCCCAGCAGCTCACATAGTGTATAGAGAGTAAAGGA Kuwana R, et al. (2003): NB DB PE
SigK Promoter -45:+11 1253314..1253369 ATGTTGGAAAAGCACATTGTATTATGTGCATTCATACAATACCGTATTACTCCGCC Kuwana R, et al. (2003): NB DB PE

Terminator

Terminator sequence Absolute position Position from stop codon Free energy
[kcal/mol]		 Downstream of
AAGATGTATTGAGGAAGTACGGGTTATAACCCGTACTTCCTTGCCTTGTGTTTATTTTTA
          >>>>>>>>>>>>>>    <<<<<<<<<<<<<<
1253261..1253292
9..40
-26.7
 yjcZ
TGGATAATAAATAAGTGGATGGCCTGGCTGACTCACTGCAGCGGGCCTTTTTTTACAGTAAA
                    >>>>>>>>>>        <<<<<<<<<
1253646..1253672
394..420
-15.3
 yjcZ

Overview



Upper Region
Copyright: Human Genome Center, Inst. Med. Sci., Univ. Tokyo; 1999-2008
Contact: Kenta Nakai