Regulated Operon: yqhV-spoIIIAABCDEFGH

Genes
Genes Synonyms Direction Genome position Function COG ID Conserved groups
yqhV yqgE - 2537689..2537970

yqgE-BAC  
spoIIIAA
- 2536690..2537613
COG3854S  
spoIIIAB
- 2536181..2536696

 
spoIIIAC
- 2535952..2536158

spoIIIAC-BAC  
spoIIIAD
- 2535544..2535945

spoIIIAD-BAC  
spoIIIAE
- 2534326..2535525

 
spoIIIAF
- 2533692..2534312

 
spoIIIAG
- 2533010..2533699

 
spoIIIAH
- 2532353..2533009

 

Operon evidence: transcriptional fusions; Northern blotting (5.9 kb transcript)
Reference: Illing N & Errington J (1991), Yoshida K, et al. (2003), Genbank U35252
Comments: Transcriptional fusions showed that spoIIIA contains at least the A, B, and C genes. Northern blotting revealed a yqhV-spoIIIAABCDEFGH transcript. The promoter in front of spoIIIAA may therefore be an internal promoter. BSORF lists a spoIIIAABCDEFGH transcript, in addition to shorter spoIIIAGH and spoIIIAH transcripts.

Promoters

Binding
factor		 Regulation Location Absolute position Binding seq.(cis-element) Experimental evidence
YlbO Negative ND ND ND Eichenberger P, et al. (2004): AR DB RG
SigE Promoter -55:+3 2537640..2537697 CATAAATAAAGAGAGGCTTGTCATAAAGTCTGCCTCACATCATACATTTTAAAGAAGC Illing N & Errington J (1991): RG DB PE OV
SpoIIID Negative -55:+3 2537640..2537697 CATAAATAAAGAGAGGCTTGTCATAAAGTCTGCCTCACATCATACATTTTAAAGAAGC Eichenberger P, et al. (2004): FT AR
SigE Promoter -40:+14 2534009..2534062 AAATAGAAATACAAGCTTCCCAGCGCGCATATATTCTAGAAGAAATGGCTGTCC Stragier P (2003): personal communication: PE RG
Guillot C, et al. (2007): PE
SpoIIID Negative -40:+14 2534009..2534062 AAATAGAAATACAAGCTTCCCAGCGCGCATATATTCTAGAAGAAATGGCTGTCC Guillot C, et al. (2007): DB,RG,PE,HM

Terminator

Terminator sequence Absolute position Position from stop codon Free energy
[kcal/mol]		 Downstream of
AAAAAGCCCGCTAAACAAGCGGGCTTTTTGCGTTGCTGT
     >>>>>>>     <<<<<<<
2532319..2532337
16..34
-15.5
 spoIIIAH

Overview



Upper Region
Copyright: Human Genome Center, Inst. Med. Sci., Univ. Tokyo; 1999-2008
Contact: Kenta Nakai