Regulated Operon: gntRKPZ

Genes
Genes Synonyms Direction Genome position Function COG ID Conserved groups
gntR
+ 4113417..4114148
COG1802K gntR-BAC x0770-BAC x1154-BAC x1448-BAC  
gntK
+ 4114141..4115682
COG1070G gntK-STA  
gntP
+ 4115711..4117057
COG2610GE gntP-BAC  
gntZ
+ 4117080..4118486
COG0362G  

Operon evidence: S1 nuclease mapping; Northern blotting (5.5 kb transcript)
Reference: Fujita Y, et al. (1986), Yoshida K, et al. (2000)
Comments: Main termination site, as determined by S1 nuclease mapping, is at position 4117549 (first T in the T stretch TTTTTTTCTT). Fujita et al. show that two internal promoters exist, for unknown sigma factor, upstream of gntZ. Yoshida shows a monocistronic 2.0 kb gntZ transcript.

Promoters

Binding
factor		 Regulation Location Absolute position Binding seq.(cis-element) Experimental evidence
CcpA Negative +138:+160 4113520..4113542 TGATTGAAAGCGGTACCATTTTA Miwa Y, et al. (1993): SDM S1 HB
Chauvaux S, et al. (1998): DB RG
Miwa Y, et al. (2000): RG
Kim JH, et al. (2005): FT
CcpB Negative ND ND ND Chauvaux S, et al. (1998): DB RG
GntR Negative -10:+12 4113373..4113394 ATACTTGTATACAAGTATACTC Yoshida K, et al. (1995): SDM GS FT
SigA Promoter -47:+16 4113336..4113398 ATGAAAGTGTTTGCATAAAAGAAATATTCACGTTATCATACTTGTATACAAGTATACTCCTTG Fujita Y, et al. (1986): S1
SigA Promoter -42:+26 4117012..4117079 CGTTGCTGGACTGGGATTCATTTTATTGTTAAGTTTAGTTGTATGAAATTAAGAAGGAGCTGTAACAC Fujita Y, et al. (1986): S1
unknown Promoter -49:+19 4117012..4117079 CGTTGCTGGACTGGGATTCATTTTATTGTTAAGTTTAGTTGTATGAAATTAAGAAGGAGCTGTAACAC Fujita Y, et al. (1986): S1

Terminator

Terminator sequence Absolute position Position from stop codon Free energy
[kcal/mol]		 Downstream of
AAAAACACGGTCAGTTTCAACTGAACCGTGTTTTTTTCTTCTATC
     >>>>>>>>>>    <<<<<<<<<<<
4118500..4118524
14..38
-14.2
 gntZ

Overview



Upper Region
Copyright: Human Genome Center, Inst. Med. Sci., Univ. Tokyo; 1999-2008
Contact: Kenta Nakai