Regulated Operon: nasBCDEF

Genes
Genes Synonyms Direction Genome position Function COG ID Conserved groups
nasB nasBA - 360442..362757
COG1251C  
nasC nasBB - 358303..360435
COG3383R  
nasD nirB - 355764..358181
COG1251C nirB-BAC  
nasE nirD - 355412..355732
COG2146PR  
nasF nasBE - 353900..355351
COG0007H  

Operon evidence: Northern blotting (9.2 kb transcript)
Reference: Nakano MM, et al. (1995), Nakano MM, et al. (1998), Yoshida K, et al. (2003)
Comments: The internal promoter in front of nasD leads to a 4.5 kb transcript

Promoters

Binding
factor		 Regulation Location Absolute position Binding seq.(cis-element) Experimental evidence
GlnR Negative -58:-42 362838..362856 GTGTAAGTTTTTGTGACAC Nakano MM, et al. (1995): DP SDM
SigA Promoter -49:+17 362781..362846 TTGTGACACGTTTAATGCGTTAACAATGCATTGTGACATAATTTTTAATAGGAGAAAACTTACGAG Nakano MM, et al. (1995): PE
TnrA Positive -58:-42 362838..362856 GTGTAAGTTTTTGTGACAC Nakano MM, et al. (1995): DP SDM
Yoshida K, et al. (2003): AR HM GS
ResD Positive -83:-39 358251..358294 TAAAATTTTTAGAACTTTTCGTATATTTTGTTACATTTTATAAC Nakano MM, et al. (1998): DB
Nakano MM, et al. (2001): RG
unknown Promoter -50:+6 358206..358261 CATTTTATAACATGTATCTTAAATATTCCTTTTTGCTGTGCTACGCTGGAAAAAAC Nakano MM, et al. (1998): PE RG
TnrA Positive -55:-39 358250..358266 TGTTACATTTTATAACA Nakano MM, et al. (1998): RG DB
Yoshida K, et al. (2003): AR HM GS
ArfM Positive ND ND ND Marino M, et al. (2001): RG DB
NsrR Negative ND ND ND Nakano MM, et al. (2006): RG
Fur Negative ND 355733..355782 TGTCGTGACCACATCATAATGACAAAAACTATCATTGAGGAGGAATGGAC Baichoo N, et al. (2002): FT

Terminator

Terminator sequence Absolute position Position from stop codon Free energy
[kcal/mol]		 Downstream of
GCAAGCAGCCCTTTTCCTCAAGGGCTGTTTTATTTATGCACC
     >>>>>>>>      <<<<<<<<
353871..353892
8..29
-13.7
 nasF

Overview



Upper Region
Copyright: Human Genome Center, Inst. Med. Sci., Univ. Tokyo; 1999-2008
Contact: Kenta Nakai