Regulated operon: phoPR

Regulated Operon:

phoPR

Genes

Genes	Synonyms	Direction	Genome position	Function	COG ID	Conserved groups
phoP		-	2977800..2978522		COG0745TK	phoP-BAC phoP-STA x1129-BAC
phoR		-	2976068..2977807		COG5002T

Operon evidence:	Northern blotting (2.7 kb transcript)
Reference:	Seki T, et al. (1988), Pragai Z, et al. (2004)
Comments:

Promoters

Binding factor	Regulation	Location	Absolute position	Binding seq.(cis-element)	Experimental evidence
CcpA	Negative	`+5:+34`	2978664..2978693	`TTTACTATAAATGAAAGCGCTATCATAAAC`	Puri-Taneja A, et al. (2005): DB RG SDM GS FT
PhoP	Positive	ND	2978727..2978755	`TGTCATGAAAGTATTATCCTAATAAAAAG`	Paul S, et al. (2004): FT RG
PhoP	Positive	ND	2978619..2978672	`ATCATAAACGTCTTTATTTCTTTTAAAAATGATGTAAAAGGCGAATTGTCGGAA`	Pragai Z, et al. (2004): FT GS Paul S, et al. (2004): FT RG
PhoP	Positive	ND	2978561..2978600	`ATAAAATCATGAAACATGTTAAGATGACATAAAATAGAGA`	Pragai Z, et al. (2004): FT GS
PhoP	Positive	ND	2978455..2978483	`ATTGTTACTCTTTTACAGTACAATTTGGA`	Paul S, et al. (2004): FT RG
ResD	Positive	ND	ND	ND	Sun G, et al. (1996): DB
SigA	Promoter	`-40:+4`	2978588..2978631	`CGAATTGTCGGAATGTCCTGCTTTCGCTAAAATAAAATCATGAA`	Pragai Z, et al. (2004): NB PE Paul S, et al. (2004): PE, in-vitro reconstitution
SigA	Promoter	`-39:+6`	2978566..2978610	`TTTCGCTAAAATAAAATCATGAAACATGTTAAGATGACATAAAAT`	Pragai Z, et al. (2004): NB PE Paul S, et al. (2004): PE, in-vitro reconstitution
unknown	Promoter	`-47:+5`	2978612..2978663	`GTCTTTATTTCTTTTAAAAATGATGTAAAAGGCGAATTGTCGGAATGTCCTG`	Paul S, et al. (2004): PE
SigA	Promoter	`-46:+10`	2978688..2978743	`ATTATCCTAATAAAAAGAGAGAAAGGCTTGCTTAATACAGCCTTTCTCTTTTTACT`	Puri-Taneja A, et al. (2005): PE, in-vitro reconstitution
SigB	Promoter	`-38:+8`	2978538..2978583	`GTTAAGATGACATAAAATAGAGAAATAGGATGTCGGGGAATTATAA`	Paul S, et al. (2004): PE, In-vitro reconstitution, DB (unclear result)
SigE	Promoter	`-43:+7`	2978554..2978603	`AAAATAAAATCATGAAACATGTTAAGATGACATAAAATAGAGAAATAGGA`	Paul S, et al. (2004): PE, In-vitro reconstitution, DB
ScoC	Negative	`-237:-193`	2978716..2978759	`AAAATGTCATGAAAGTATTATCCTAATAAAAAGAGAGAAAGGCT`	Kaushal B, et al. (2010): PE,FT
ScoC	Negative	`-87:-66`	2978588..2978609	`TTCGCTAAAATAAAATCATGAA`	Kaushal B, et al. (2010): PE,FT
ScoC	Negative	`-56:-6`	2978528..2978578	`GATGACATAAAATAGAGAAATAGGATGTCGGGGAATTATAATACTGGAGGC`	Kaushal B, et al. (2010): PE,FT

Terminator

Terminator sequence	Absolute position	Position from stop codon	Free energy [kcal/mol]	Downstream of
`TTGACGGCGGGAACCTATTTGTGTTCCCGTCCTTTTTTGTGTCTTCT >>>>>>>>> <<<<<<<<<`	2975949..2975974	94..119	-16.3	phoR

Overview

Upper Region

Copyright: Human Genome Center, Inst. Med. Sci., Univ. Tokyo; 1999-2008
Contact: Kenta Nakai