Regulated Operon: | skf |
Genes | Synonyms | Direction | Genome position | Function | COG ID | Conserved groups |
---|---|---|---|---|---|---|
ybcO | skfA | + | 213926..214093 | |||
ybcP | ybcQ, skfB, ybcQ | + | 214160..215392 | |||
ybcS | skfC | + | 215389..215946 | |||
ybcT | skfD | + | 215943..216878 | |||
ybdA | skfE | + | 216897..217616 | |||
ybdB | skfF | + | 217681..219027 | |||
ybdD | skfG | + | 219074..219487 | |||
ybdE | skfH | + | 219593..220018 |
Operon evidence: | Northern blotting (6.5 kb transcript) |
---|---|
Reference: | Gonzalez-Pastor JE, et al. (2003), Allenby NEE, et al. (2006) |
Comments: | Northern blotting during during phosphate limitation showed an intense 0.25 kb skfA-specific transcript, and a weaker 6.5 kb skfABCDEFGh transcript. The skfA transcript is stabilized by a stem-loop structure in the skfA-skfB intergenic region. |
Binding factor |
Regulation | Location | Absolute position | Binding seq.(cis-element) | Experimental evidence |
---|---|---|---|---|---|
AbrB | Negative | ND | ND | ND |
Fujita M, et al. (2005): RG |
PhoP | Positive | ND | ND | ND |
Allenby NEE, et al. (2006): DB RG NB GS |
SigA | Promoter | -64:+4 | 213824..213891 | AATTTTTAGGATAATATACAAAATCCCCCTTACTTCGACAATTGCAATCTGGTATTATCGTATCGCAT |
Chen G, et al. (2006): PE |
Spo0A | Positive | -64:+4 | 213824..213891 | AATTTTTAGGATAATATACAAAATCCCCCTTACTTCGACAATTGCAATCTGGTATTATCGTATCGCAT |
Molle V, et al. (2003): GS CH Fujita M, et al. (2005): RG OV, gfp fusion, GS Chen G, et al. (2006): FT, binding site mutation |
Terminator sequence | Absolute position | Position from stop codon | Free energy [kcal/mol] |
Downstream of |
---|---|---|---|---|
TAACATTTGAGAATAGGGAGTTGAGCGTATTTGCTTATACTCCTTATTTTCTCTTAAG >>>>>>>>>> <<<<<<<<<<< |
ybcO | |||
ATGAAACGGTCCTTCCTATAAGCAGGAAGGTTTTTTAATGAAATA >>>>>>> <<<<<<< |
ybdE |
|