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Copyright: Human Genome Center, Inst. Med. Sci., Univ. Tokyo; 1999-2006
Contact: Kenta Nakai |
| Regulated Operon: | bglPH-yxiE |
| Genes | Synonyms | Direction | Genome position | Function | COG ID | Genus clusters |
|---|---|---|---|---|---|---|
| bglP | sytA | - | 4032796..4034625 | phosphotransferase system (PTS) beta-glucoside-specific enzyme IIBCA component | COG1264 | |
| bglH | - | 4031364..4032773 | beta-glucosidase | |||
| yxiE | - | 4030815..4031261 | COG0589 |
| Operon evidence: | Northern blotting (4.0 kb transcript) |
|---|---|
| Reference: | Le Coq D, et al. (1995), Kruger S & Hecker M (1995), Kruger S, et al. (1996), Yoshida K, et al. (2000), Antelmann H, et al. (2000) |
| Comments: | Internal promoter in front of yxiE, leading to a 0.5 kb transcript. In the presence of beta-glucosides, LicT binds to the ribonucleic antiterminator (RAT) sequence just upstream of the transcriptional terminator downstream of the bglP promoter, alleviating termination at this stem-loop. |
| Binding factor |
Regulation | Location | Absolute position | Binding seq.(cis-element) | Experimental evidence |
|---|---|---|---|---|---|
| CcpA | Negative | -47:+3 | 4034832..4034881 | AAAATGAAAGCGTTGACATCTCACGAATCTAGTGCTAAAGTATACCTACA |
Kruger S, et al. (1996): RO DP |
| LicT | Positive | +25:+57 | 4034778..4034810 | GGATTGTTACTGCGAAAGCAGGCAAAACCTAAA |
Kruger S & Hecker M (1995): RG DB Kruger S, et al. (1996): RO DP |
| SigA | Promoter | -47:+3 | 4034832..4034881 | AAAATGAAAGCGTTGACATCTCACGAATCTAGTGCTAAAGTATACCTACA |
Le Coq D, et al. (1995): PE |
| Terminator sequence | Absolute position | Position from stop codon | Free energy [kcal/mol] |
Downstream of |
|---|---|---|---|---|
| AAAAAGCGCCCTTGGGCGCTTTTTTTTATTTAGA >>>>> <<<<< |
yxiE | |||
| CCTAAATGGTGCAGGTGCAGGAGGAGACTCTCGCAATCTGTGTCATTTAGGTTTTTTTAT >>>>>>>>>>>>>>>>> <<<<<<<<<<<<<<<<<< |
bglP |
|