Regulated Operon: cotVWX

Genes
Genes Synonyms Direction Genome position Function COG ID Genus clusters
cotV
- 1250943..1251329 spore coat protein (insoluble fraction)
 
cotW
- 1250585..1250902 spore coat protein (insoluble fraction)
 
cotX
- 1249968..1250486 spore coat protein (insoluble fraction)
 

Operon evidence: Northern blotting (1.6 kb transcript)
Reference: Zhang J, et al. (1994), Zhang J, et al. (1993)
Comments: The internal promoter in front of cotX leads to a 0.6 kb transcript.


Promoters

Binding
factor
Regulation Location Absolute position Binding seq.(cis-element) Experimental evidence
GerE Positive -53:-32 1251385..1251406 AAATTGGTTATTTTTATTATTC Zhang J, et al. (1994): FT RO
SigK Promoter -42:+6 1251348..1251395 TTTTATTATTCCGCTCTGCACCCCATTTGCATTATATAGAGTATGGAT Zhang J, et al. (1994): NB PE DB RO
GerE Positive -70:-50 1250557..1250577 TAAAAAATAGGGTTCTTCATC Zhang J, et al. (1994): FT RO
GerE Positive -50:-32 1250539..1250557 CAGGATATATGACTCAGTC Zhang J, et al. (1994): FT RO
SigK Promoter -43:+5 1250503..1250550 TATGACTCAGTCAAAATAAGAGGCTCGCTCATTTAATAACAGTAAAAG Zhang J, et al. (1994): NB PE DB RO
SpoIIID Negative -35:-7 1250513..1250542 AGTCAAAATAAGAGGCTCGCTCATTTAATA Ichikawa H, et al. (2000): GS FT

Terminator

Terminator sequence Absolute position Position from stop codon Free energy
[kcal/mol]
Downstream of
TAAAAGCAGAGCTAAAAACGCTCTGCTTTTTCTTATTTTCC
     >>>>>>>       <<<<<<<
1249938..1249958
10..30
-13.5
cotX

Overview



Upper Region






Copyright: Human Genome Center, Inst. Med. Sci., Univ. Tokyo; 1999-2006
Contact: Kenta Nakai