Regulated Operon: deoR-dra-nupC-pdp

Genes
Genes Synonyms Direction Genome position Function COG ID Genus clusters
deoR yxxC - 4051396..4052337 transcriptional regulator COG2390 x0511-BAC  
dra
- 4050655..4051290 deoxyribose-phosphate aldolase COG0274 dra-BAC-1 dra-BAC-2  
nupC
- 4049358..4050539 pyrimidine-nucleoside transport protein COG1972 nupC-BAC-1 nupC-STA x1697-BAC  
pdp
- 4048027..4049328 pyrimidine-nucleoside phosphorylase
pdp-BAC  

Operon evidence: Northern blotting (4.5 kb transcript)
Reference: Yoshida K, et al. (2000), Yoshida K, et al. (1995), Saxild HH, et al. (1996)
Comments: A 3.4 kb dra-nupC-pdp transcript was also detected, as well as a monocistronic (1.0 kb) dra transcript terminating at the putative readthrough terminator downstream of dra. Northern blotting results by Yoshida also showed a monocistronic (0.8 kb) deoR transcript.


Promoters

Binding
factor
Regulation Location Absolute position Binding seq.(cis-element) Experimental evidence
CcpA Negative +62:+84 4051238..4051260 GCTTTGAAACCGCATACACAAAA Miwa Y, et al. (2000): RG
DeoR Negative -60:-22 4051341..4051379 ATTGAACAAAATTTCAATTACCAATTTACATATGTTCAA Zeng X, et al. (2000): GS FT
Zeng X & Saxild HH (1999): DP RG DB SDM GS
Saxild HH, et al. (1996): DB HM
SigA Promoter -60:+12 4051310..4051379 ATTGAACAAAATTTCAATTACCAATTTACATATGTTCAAAAGTCGGTTATGCTAAAAAATATCTTAACAA Saxild HH, et al. (1996): PE

Terminator

Terminator sequence Absolute position Position from stop codon Free energy
[kcal/mol]
Downstream of
ATAAAGCACATCCCATGCTGAGCGGGGTGTGCTTTTTTAATTATAGG
     >>>>>>>>>         <<<<<<<<<
4047992..4048018
9..35
-17.2
pdp
GCTGACGGAAGGCAGACTGCTTTCCGTTTTCTGAGGAAACA
     >>>>>>>>>   <<<<<<<<<
4050592..4050612
43..63
-14.9
dra

Overview



Upper Region






Copyright: Human Genome Center, Inst. Med. Sci., Univ. Tokyo; 1999-2006
Contact: Kenta Nakai