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Copyright: Human Genome Center, Inst. Med. Sci., Univ. Tokyo; 1999-2006
Contact: Kenta Nakai |
| Regulated Operon: | dnaAN |
| Genes | Synonyms | Direction | Genome position | Function | COG ID | Genus clusters |
|---|---|---|---|---|---|---|
| dnaA | dnaH, dnaJ, dnaK | + | 410..1750 | COG0593 | dnaA-BAC dnaA-STA dnaA-STR dnaJ-BAC dnaJ-STA dnaJ-STR dnaK-BAC dnaK-MYC dnaK-STA | |
| dnaN | dnaG, dnaK | + | 1939..3075 | DNA polymerase III (beta subunit) | COG0592 | dnaN-BAC dnaN-STA dnaN-STR x1222-CLO dnaG-OYP-1 dnaG-OYP-2 dnaG-STA dnaG-STR dnaK-BAC dnaK-MYC dnaK-STA |
| Operon evidence: | Northern blotting (2.9 kb transcript); transcriptional experiments |
|---|---|
| Reference: | Ogura Y, et al. (2001), Moriya S, et al. (1988), Moriya S, et al. (1985), Ogasawara N, et al. (1985), BSORF |
| Comments: | Readthrough terminator downstream of dnaA, leading to a 1.7 kb transcript. The earlier result (Ogasawara et al., 1985) showing an internal promoter in front of dnaN is incorrect (Ogura et al., 2001; Ogasawara, personal communication). The Northern blotting experiments listed in BSORF suggest that readthrough may occur at the terminator downstream of dnaN. |
| Binding factor |
Regulation | Location | Absolute position | Binding seq.(cis-element) | Experimental evidence |
|---|---|---|---|---|---|
| DnaA | Negative | -170:-147 | 4214617..10 | TCACGCATGTGGATATCTTTTTCG |
Fukuoka T, et al. (1990): FT (medium) |
| DnaA | Negative | -146:-120 | 11..37 | GCTTTTTTTAGTATCCACAGAGGTTAT |
Fukuoka T, et al. (1990): FT (medium) |
| DnaA | Negative | -133:-107 | 24..50 | TCCACAGAGGTTATCGACAACATTTTC |
Fukuoka T, et al. (1990): FT (medium) |
| DnaA | Negative | -99:-80 | 58..77 | CAACCCCTGTGGACAAGGTT |
Fukuoka T, et al. (1990): FT (medium) |
| DnaA | Negative | -64:-36 | 93..121 | CCGCTTTGTGGATAAGATTGTGACAACCA |
Fukuoka T, et al. (1990): FT (strong) |
| DnaA | Negative | +30:+63 | 186..219 | TTCCTCTTTATCCACAAAGTGTGGATAAGTTGTG |
Fukuoka T, et al. (1990): FT (strong) |
| DnaA | Negative | +80:+97 | 246..263 | AGGTTGTCCACAAGTTGT |
Fukuoka T, et al. (1990): FT (medium) |
| DnaA | Negative | between dnaA and dnaN | 1767..1837 | TCGGGGAAAGTGTGAATAACTTTTCGGAAGTCATACACAGTCTGTCCACATGTGGATAGGCTGTGTTTCCT |
Fukuoka T, et al. (1990): FT (medium) |
| DnaA | Negative | between dnaA and dnaN | 1843..1871 | TTTCACAACTTATCCACAAATCCACAGGC |
Fukuoka T, et al. (1990): FT (strong) |
| SigA | Promoter | -47:+18 | 110..174 | TTGTGACAACCATTGCAAGCTCTCGTTTATTTTGGTATTATATTTGTGTTTTAACTCTTGATTAC |
Moriya S, et al. (1988): S1 Ogasawara N, et al. (1985): S1 |
| Terminator sequence | Absolute position | Position from stop codon | Free energy [kcal/mol] |
Downstream of |
|---|---|---|---|---|
| ACACTGCTGCCGACCCGTCGGCAGCTTTTCTATTCGGTAT >>>>>>>> <<<<<<<< |
dnaN |
|