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Copyright: Human Genome Center, Inst. Med. Sci., Univ. Tokyo; 1999-2006
Contact: Kenta Nakai |
| Regulated Operon: | gabTD |
| Genes | Synonyms | Direction | Genome position | Function | COG ID | Genus clusters |
|---|---|---|---|---|---|---|
| gabT | ycnG | + | 441139..442449 | 4-aminobutyrate aminotransferase | COG0160 | gabT-BAC |
| gabD | ycnH | + | 442518..443906 | succinate-semialdehyde dehydrogenase | COG1012 | gabD-BAC |
| Operon evidence: | lacZ gene fusion to gabT and gabTD; 5' primer extension analysis of the downstream glcU-gdh operon; Northern blotting (2.6 kb transcript) |
|---|---|
| Reference: | Belitsky BR & Sonenshein AL (2002), BSORF |
| Comments: | The lacZ fusions showed that an internal promoter (sigma factor unknown) exists between gabT and gabD. The Northern blotting results in BSORF only showed the gabTD transcript. |
| Binding factor |
Regulation | Location | Absolute position | Binding seq.(cis-element) | Experimental evidence |
|---|---|---|---|---|---|
| GabR | Positive | ND | ND | ND |
Belitsky BR & Sonenshein AL (2002): PE SDM |
| SigA | Promoter | -39:+18 | 441072..441128 | TTGGTACTTTTCATCATACCAAAGAGAAGTCAGAATGATAAGAAAATACCGACAGGG |
Belitsky BR & Sonenshein AL (2002): PE |
| SigB | Promoter | -37:+4 | 442324..442364 | AACGGATTACTTTTGCTGACAGCGGGAATTAACGGTAATAT |
Petersohn A, et al. (1999): HB PE |
| Terminator sequence | Absolute position | Position from stop codon | Free energy [kcal/mol] |
Downstream of |
|---|---|---|---|---|
| CCTGAGAGCTGCCGGATTTTCCGGCAGCTCTTTTTGTGTTCCGGC >>>>>>>>>>> <<<<<<<<<<< |
gabD |
|