Regulated Operon: gabTD

Genes
Genes Synonyms Direction Genome position Function COG ID Genus clusters
gabT ycnG + 441139..442449 4-aminobutyrate aminotransferase COG0160 gabT-BAC  
gabD ycnH + 442518..443906 succinate-semialdehyde dehydrogenase COG1012 gabD-BAC  

Operon evidence: lacZ gene fusion to gabT and gabTD; 5' primer extension analysis of the downstream glcU-gdh operon; Northern blotting (2.6 kb transcript)
Reference: Belitsky BR & Sonenshein AL (2002), BSORF
Comments: The lacZ fusions showed that an internal promoter (sigma factor unknown) exists between gabT and gabD. The Northern blotting results in BSORF only showed the gabTD transcript.


Promoters

Binding
factor
Regulation Location Absolute position Binding seq.(cis-element) Experimental evidence
GabR Positive ND ND ND Belitsky BR & Sonenshein AL (2002): PE SDM
SigA Promoter -39:+18 441072..441128 TTGGTACTTTTCATCATACCAAAGAGAAGTCAGAATGATAAGAAAATACCGACAGGG Belitsky BR & Sonenshein AL (2002): PE
SigB Promoter -37:+4 442324..442364 AACGGATTACTTTTGCTGACAGCGGGAATTAACGGTAATAT Petersohn A, et al. (1999): HB PE

Terminator

Terminator sequence Absolute position Position from stop codon Free energy
[kcal/mol]
Downstream of
CCTGAGAGCTGCCGGATTTTCCGGCAGCTCTTTTTGTGTTCCGGC
     >>>>>>>>>>>   <<<<<<<<<<<
443925..443949
19..43
-24.2
gabD

Overview



Upper Region






Copyright: Human Genome Center, Inst. Med. Sci., Univ. Tokyo; 1999-2006
Contact: Kenta Nakai