Regulated Operon: glpFK

Genes
Genes Synonyms Direction Genome position Function COG ID Genus clusters
glpF
+ 1001834..1002658 glycerol uptake facilitator COG0580 glpF-BAC  
glpK
+ 1002677..1004167 glycerol kinase COG0554 glpK-BAC glpK-MYC glpK-STA  

Operon evidence: Northern blotting (2.4 kb transcript); S1 nuclease mapping of the 3' end
Reference: Holmberg C, et al. (1990), Beijer L, et al. (1993), Holmberg C & Rutberg L (1991), Holmberg C & Rutberg L (1992)
Comments: Readthrough at this terminator leads to a 4.4 kb glpFKD transcript. This terminator also functions as a terminator/anti-terminator for transcription starting from the glpD promoter. S1 nuclease mapping of the 3' mRNA end shows that transcription ends in the TGGTCTCT section just in front of the T-stretch. The antiterminator protein GlpP is thought to allow transcription to proceed, in the presence of glycerol-3-phosphate through the stemloop structure between the glpF promoter and coding sequence.

Promoters

Binding
factor		 Regulation Location Absolute position Binding seq.(cis-element) Experimental evidence
CcpA Negative -37:-19 1001650..1001668 GATTGACACCGCTTTCATG Darbon E, et al. (2002): RG FT
GlpP Positive +13:+35 1001699..1001721 TGTGATGGAGAACTCGGAGACCA Beijer L, et al. (1993): DB NB
SigA Promoter -47:+18 1001640..1001704 GACGGAAAGTGATTGACACCGCTTTCATGCACTGATACAATTGCACTAGGTTAATACATTGTGAT Holmberg C, et al. (1990): PE

Terminator

Terminator sequence Absolute position Position from stop codon Free energy
[kcal/mol]		 Downstream of
GAGGAGAGACCACAGCACCAAAGTGTAAGCATGCACTTTGGCTGTTGTGGTCTCTTTTTCTATTTACCG
     >>>>>>>>>>>>>>>>>>>>>>    <<<<<<<<<<<<<<<<<<<<<<<
1004223..1004271
56..104
-37.5
 glpK
CGGAGACCACAGCAGCTCTTTACGGCAAATGTTTATGCACCCGTAAAGCGGTTTGTTGTGGTTTTTTTATTCTCTTCTTCTCTATCATGCTTTTTAATCGTGA
 >>>>>>>>>>>>>>>>>>>>>>>>               <<<<<<<<<<<<<<<<<<<<<<<<<<
1001714..1001778
-944..-880
-30.9
 glpF

Overview



Upper Region
Copyright: Human Genome Center, Inst. Med. Sci., Univ. Tokyo; 1999-2006
Contact: Kenta Nakai