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Copyright: Human Genome Center, Inst. Med. Sci., Univ. Tokyo; 1999-2006
Contact: Kenta Nakai |
| Regulated Operon: | glpTQ |
| Genes | Synonyms | Direction | Genome position | Function | COG ID | Genus clusters |
|---|---|---|---|---|---|---|
| glpT | ybeE | - | 233982..235316 | glycerol-3-phosphate permease | COG2271 | glpT-BAC |
| glpQ | ybeD | - | 233002..233883 | glycerophosphoryl diester phosphodiesterase | COG0584 | x0792-BAC |
| Operon evidence: | Northern blotting (2.6 kb transcript) |
|---|---|
| Reference: | Nilsson RP, et al. (1994), Antelmann H, et al. (2000) |
| Comments: | The internal promoter in front of glpQ, leading to a 1.1 kb transcript. Transcriptional terminatation at the stem-loop downstream of the glpT promoter is thought to be alleviated by GlpP binding to the ribosomal antiterminator (RAT) sequence in the presence of glycerol-3-phosphate. |
| Binding factor |
Regulation | Location | Absolute position | Binding seq.(cis-element) | Experimental evidence |
|---|---|---|---|---|---|
| GlpP | Positive | +27:+49 | 235423..235445 | TTGGATGGAGATGAGGAGAGCCA |
Nilsson RP, et al. (1994): HM |
| SigA | Promoter | -45:+18 | 235454..235516 | GAAAATTACCCGTTTACATTCGCTCCGGACTATGATAATTTAAGAAAGCGCTATCATGATAAG |
Nilsson RP, et al. (1994): S1 |
| PhoP | Positive | -65:-16 | 233932..233981 | GAAAGACACATAAAAGATTAATAGTTTTCCAACACGCCGTTTACATCCGT |
Antelmann H, et al. (2000): NB DB 2D-PAGE Allenby NE, et al. (2005): DB AR |
| SigA | Promoter | -46:+10 | 233907..233962 | AATAGTTTTCCAACACGCCGTTTACATCCGTTTTTTATTATGAAAGACAGGTAGTG |
Antelmann H, et al. (2000): NB PE |
| Terminator sequence | Absolute position | Position from stop codon | Free energy [kcal/mol] |
Downstream of |
|---|---|---|---|---|
| GAATCCAACCCCTTTTGACAAGGGGTGTTTTCTGGCTCTTTT >>>>>>> <<<<<<< |
glpT | |||
| AAAAACCTTGCGACAACTATCGCAAGGTTTTCTTCTATATTT >>>>>>>> <<<<<<<< |
glpQ |
|