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Copyright: Human Genome Center, Inst. Med. Sci., Univ. Tokyo; 1999-2006
Contact: Kenta Nakai |
| Regulated Operon: | lcfA-ysiAB-etfBA |
| Genes | Synonyms | Direction | Genome position | Function | COG ID | Genus clusters |
|---|---|---|---|---|---|---|
| lcfA | - | 2917714..2919396 | long chain acyl-CoA synthetase | COG0318 | lcfA-BAC | |
| ysiA | - | 2917025..2917609 | COG1309 | ysiA-BAC | ||
| ysiB | - | 2916234..2917010 | COG1024 | |||
| etfB | - | 2915446..2916219 | electron transfer flavoprotein (beta subunit) | COG2086 | etfB-BAC x1559-CLO | |
| etfA | - | 2914433..2915410 | electron transfer flavoprotein (alpha subunit) | COG2025 | etfA-BAC |
| Operon evidence: | Northern blotting (5.1 kb transcript) |
|---|---|
| Reference: | Wipat A, et al. (1996), Raposo MP, et al. (2004), Matsuoka H, et al. (2006), Barabesi C, et al. (2007) |
| Comments: | The internal promoter in front of ysiA and read-through terminators downstream of lcfA and ysiA lead to a 1.8 kb monocistronic lcfA transcript, a 0.7 kb monocistronic ysiA transcript, and a 3.2 kb ysiAB-etfBA transcript. |
| Binding factor |
Regulation | Location | Absolute position | Binding seq.(cis-element) | Experimental evidence |
|---|---|---|---|---|---|
| CcpA | Negative | ND | 2918966..2918988 | AAAATGAAAACGTTATCAATAGT |
Miwa Y, et al. (2000): RG |
| YsiA | Negative | -43:+24 | 2917630..2917696 | GAATAAACTTGACAAACCTGAAAATCCTTCTTATCATGAAATTATGAATGAATAGTCATTCATTTCT |
Matsuoka H, et al. (2006): NB DB PE RG FT RG |
| SigA | Promoter | -43:+24 | 2917630..2917696 | GAATAAACTTGACAAACCTGAAAATCCTTCTTATCATGAAATTATGAATGAATAGTCATTCATTTCT |
Matsuoka H, et al. (2006): PE NB |
| Terminator sequence | Absolute position | Position from stop codon | Free energy [kcal/mol] |
Downstream of |
|---|---|---|---|---|
| GAAATCCCGGACTTTAAAAGTCCGGGTTTTTTCATATTTAT >>>>>>>>> <<<<<<<<< |
etfA |
|