 |
Copyright: Human Genome Center, Inst. Med. Sci., Univ. Tokyo; 1999-2006
Contact: Kenta Nakai |
| Regulated Operon: | lepA-hemN-hrcA-grpE-dnaKJ-yqeTUV |
| Genes | Synonyms | Direction | Genome position | Function | COG ID | Genus clusters |
|---|---|---|---|---|---|---|
| lepA | yqxB, yqeQ | - | 2630133..2631971 | GTP-binding protein | COG0481 | lp_2015-LAB lp_2015-STA x0099-CLO |
| hemN | yqeR | - | 2628980..2630080 | coproporphyrinogen III oxidase | COG0635 | x1621-STA |
| hrcA | yqxE, yqeS | - | 2627829..2628860 | transcriptional regulator | COG1420 | hrcA-BAC hrcA-STA hrcA-STR |
| grpE | - | 2627194..2627757 | heat-shock protein | COG0576 | grpE-STA | |
| dnaK | - | 2625335..2627170 | class I heat-shock protein (molecular chaperone) | COG0443 | dnaK-BAC dnaK-MYC dnaK-STA | |
| dnaJ | - | 2624017..2625135 | heat-shock protein | COG0484 | dnaJ-BAC dnaJ-STA dnaJ-STR | |
| yqeT | - | 2623057..2623992 | COG2264 | yqeT-BAC | ||
| yqeU | - | 2622264..2623034 | COG1385 | |||
| yqeV | - | 2620909..2622264 | COG0621 |
| Operon evidence: | Northern blotting (8.0 kb hrcA-grpE-dnaKJ-yqeTUV transcript and a 7.0 kb lepA-hemN-hrcA-grpE-dnaK transcript) |
|---|---|
| Reference: | Schulz A, et al. (1994), Homuth G, et al. (1996), Hippler B, et al. (1997), Homuth G, et al. (1997), Condon C, et al. (2002), Genbank D83717, Genbank X52064 |
| Comments: | Readthrough terminators downstream of lepA and dnaK lead to a 1.8 kb monocistronic lepA transcript and a 3.6 kb hrcA-grpE-dnaK transcript, respectively. The internal promoter upstream of dnaJ produces to a 4.3 kb dnaJ-yqeTUV transcript. A cleavage site between hrcA and grpE produced a 7.0 kb grpE-dnaKJ-yqeTUV mRNA and a 2.6 kb grpE-dnaK mRNA molecule. Slot-blot and immunoblot experiments with a lepA mutant showed that lepA and hemN are transcribed from the same promoter. |
| Binding factor |
Regulation | Location | Absolute position | Binding seq.(cis-element) | Experimental evidence |
|---|---|---|---|---|---|
| SigA | Promoter | -58:+12 | 2631997..2632066 | ACTTTTCTCTTTCTTGTTTTACATTGAATCTTTACAATCCTATTGATATAATCTAAGCTAGTGTATTTTG |
Hippler B, et al. (1997): PE |
| ArfM | Positive | ND | ND | ND |
Homuth G, et al. (1999): RG PE |
| HrcA | Negative | +5:+31 | 2628875..2628901 | TTAGCACTCGCTTATTGAGAGTGCTAA |
Zuber U, et al. (1994): SDM HB Voelker U, et al. (1994): 2D PAGE, HB |
| SigA | Promoter | -49:+18 | 2628888..2628954 | TGGGTGAGTTATAATTGACATTTTTCTTGTGGTTTGATACTTTTGTTATAGAATTAGCACTCGCTTA |
Wetzstein M, et al. (1992): PE NB Homuth G, et al. (1997): NB |
| unknown | Promoter | -49:+8 | 2629035..2629091 | CGGCCGTTCCCTGGATGGACTTTTCCCAAGCGTGTTAAAAGACCTCGCTGAAAAAGG |
Wetzstein M, et al. (1992): PE |
| unknown | Promoter | -52:+8 | 2627771..2627830 | AAGGGAATTTTGGCAAATTTTATCGAAGGGCAGCACCTGTCCTTCTCCTTACACTTTGAG |
Wetzstein M, et al. (1992): PE Voelker U, et al. (1994): 2D PAGE |
| SigA | Promoter | ND | 2625156..2625208 | AAAGGAATTGAAAAATCATAATTCAAAATGATACAATCTAATTTATGTGAGAA |
Homuth G, et al. (1997): NB |
| Terminator sequence | Absolute position | Position from stop codon | Free energy [kcal/mol] |
Downstream of |
|---|---|---|---|---|
| AAAAATCGGTGCATTAAAATGTACCGATTTTTTTATTTAGCC >>>>>>>>> <<<<<<<<< |
yqeV | |||
| AGAAAGTCAAAGTCAGGCATCTCTTGGCTTTGACTTTTTTTCTTGCCCGGG >>>>>>>>>>>> <<<<<<<<<<<< |
dnaK | |||
| TCCGAAAAAACAATAGAAGCCGCCGCAGTCTTGTACTGCGGCTTCTTTCTGTAAAGA >>>>>>>> <<<<<<<< |
lepA |
|