Regulated Operon: narGHJI

Genes
Genes Synonyms Direction Genome position Function COG ID Genus clusters
narG
- 3825282..3828968 nitrate reductase (alpha subunit) COG5013 narG-BAC  
narH
- 3823829..3825292 nitrate reductase (beta subunit) COG1140  
narJ
- 3823249..3823803 nitrate reductase (protein J) COG2180 narJ-BAC  
narI
- 3822581..3823252 nitrate reductase (gamma subunit) COG2181 narI-BAC  

Operon evidence: Northern blotting (6.4 kb transcript)
Reference: Cruz Ramos H, et al. (1995), Reents H, et al. (2006), Genbank Z49884
Comments:


Promoters

Binding
factor
Regulation Location Absolute position Binding seq.(cis-element) Experimental evidence
FNR Positive -53:-24 3829024..3829053 AGTGTGTGACATAGTTCACAAGGAAACACG Cruz Ramos H, et al. (1995): DB PE
Reents H, et al. (2006): DB NB AR RG SDM
SigA Promoter -53:+14 3828987..3829053 AGTGTGTGACATAGTTCACAAGGAAACACGCTGTCTCTGTTTATAGTGAACTTAGCATCACCTGACT Cruz Ramos H, et al. (1995): PE
Reents H, et al. (2006): PE

Terminator

Terminator sequence Absolute position Position from stop codon Free energy
[kcal/mol]
Downstream of
AAAATCCCCCTTGCAAGAGGGGGTTTTTTCTGCCAAGG
     >>>>>>>    <<<<<<<
3822559..3822576
5..22
-15.9
narI

Overview



Upper Region






Copyright: Human Genome Center, Inst. Med. Sci., Univ. Tokyo; 1999-2006
Contact: Kenta Nakai