Regulated Operon: rsbRSTUVW-sigB-rsbX

Genes
Genes Synonyms Direction Genome position Function COG ID Genus clusters
rsbR ycxR + 518969..519793
COG1366  
rsbS ycxS + 519798..520163 antagonist of RsbT COG1366  
rsbT ycxT + 520167..520568 switch protein/serine-threonine kinase COG2172  
rsbU
+ 520580..521587 serine phosphatase COG2208 rsbU-STA x0328-BAC  
rsbV
+ 521649..521978 anti-anti-sigma factor (antagonist of RsbW) COG1366 rsbV-BAC rsbV-STA  
rsbW
+ 521975..522457 switch protein/serine kinase and anti-sigma factor (inhibitory sigma-B binding protein) COG2172  
sigB rpoF + 522417..523211 RNA polymerase sigma-37 factor (sigma-B) COG1191  
rsbX
+ 523211..523810 serine phosphatase
 

Operon evidence: S1 nuclease mapping of 3' end; lacZ fusion to rsbV; the promoter in front of rsbR was the only promoter detected in the rsbRSTU region
Reference: Kalman S, et al. (1990), Wise AA & Price CW (1995)
Comments: The S1 nuclease mapping showed that transcription ends in the T-stretch of the indicated terminator (positions 523844..523849). Internal promoter in front of rsbV.

Promoters

Binding
factor		 Regulation Location Absolute position Binding seq.(cis-element) Experimental evidence
SigA Promoter -45:+21 518878..518943 AGAGCAACTTTTTTTGTTTTCAAAAAACATAAACGATATAATAGTGAAATAACGAAAAAATATGTT Wise AA, et al. (1995): PE
SigB Promoter -40:+3 521577..521619 GAAAGGTTTAACGTCTGTCAGACGAGGGTATAAAGCAACTAGT Kalman S, et al. (1990): PE S1 DB RG
Boylan SA, et al. (1993): PE; Western blot; RG
Voelker U, et al. (1994): 2D PAGE, HB

Terminator

Terminator sequence Absolute position Position from stop codon Free energy
[kcal/mol]		 Downstream of
AAAAAGAAGCTGGACATCCGGCTTCTTTTTTTTGCGGTTG
     >>>>>>>>    <<<<<<<<
523824..523843
14..33
-13.5
 rsbX

Overview



Upper Region
Copyright: Human Genome Center, Inst. Med. Sci., Univ. Tokyo; 1999-2006
Contact: Kenta Nakai