Regulated Operon: sacXY

Genes
Genes Synonyms Direction Genome position Function COG ID Genus clusters
sacX ipa-14r, sacS + 3941255..3942634 negative regulatory protein of SacY COG1263  
sacY ipa-13r, sacS + 3942688..3943530 transcriptional antiterminator COG3711  

Operon evidence: lacZ gene fusion
Reference: Zukowski MM, et al. (1990), Glaser P, et al. (1990), Crutz AM & Steinmetz M (1992)
Comments: Binding of SacT (at low sucrose concentration) and by both SacT and SacY (at higher concentrations) to the ribonucleic antiterminator (RAT) sequence prevents formation of the terminator in the sacXY promoter, allowing transcription of the downstream sequence.

Promoters

Binding
factor		 Regulation Location Absolute position Binding seq.(cis-element) Experimental evidence
DegU Positive -112:-56 3940856..3940912 GTCTTAAAGGTTTTTTTCATTCTAAGAACACCACACACAACCTTTTTCCCATCCATT Crutz AM & Steinmetz M (1992): DB RG DP
SacT Positive +19:+61 3940986..3941028 TATGGCGGGATTGTGACTGGGCAGGCAGGCAAGACCCAATGAT Tortosa P, et al. (1995): RG SDM DP
SacY Positive +19:+61 3940986..3941028 TATGGCGGGATTGTGACTGGGCAGGCAGGCAAGACCCAATGAT Tortosa P, et al. (1995): RG SDM DP
SigA Promoter -48:+20 3940920..3940987 CTTTTCATACTATTGCTATACAGCCATGAACAGCATAAAATGAACGTTATTACAGTTATCACCACATA Crutz AM & Steinmetz M (1992): PE

Terminator

Terminator sequence Absolute position Position from stop codon Free energy
[kcal/mol]		 Downstream of
AAAAACGCTTTTGATCATCTCAAAAGCGTTTTTTTATCTGATT
     >>>>>>>>>     <<<<<<<<<
3943544..3943566
14..36
-13.4
 sacY
ATTCTCATGCTCCGTCGTCGACGCGGGCATTTTTGTCATTTTCA
       >>>>>>>>>   <<<<<<<<<<
3941123..3941144
-1511..-1490
-13.1
 sacX

Overview



Upper Region
Copyright: Human Genome Center, Inst. Med. Sci., Univ. Tokyo; 1999-2006
Contact: Kenta Nakai