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Copyright: Human Genome Center, Inst. Med. Sci., Univ. Tokyo; 1999-2006
Contact: Kenta Nakai |
| Regulated Operon: | spo0A |
| Genes | Synonyms | Direction | Genome position | Function | COG ID | Genus clusters |
|---|---|---|---|---|---|---|
| spo0A | spo0C, spo0G, spoIIL, sof-1 | - | 2517257..2518060 | two-component response regulator | COG0784 | spo0A-BAC |
| Operon evidence: | S1 nuclease mapping; Northern blotting (950 bp transcript from SigA promoter; 800 bp transcript from SigH promoter) |
|---|---|
| Reference: | Ferrari FA, et al. (1985), Eymann C, et al. (2001), BSORF |
| Comments: | The S1 nuclease mapping experiment showed that transcription terminates at the seventh nucleotide in the T-stretch TTTTGTTTTGGGG. |
| Binding factor |
Regulation | Location | Absolute position | Binding seq.(cis-element) | Experimental evidence |
|---|---|---|---|---|---|
| SigA | Promoter | -43:+15 | 2518244..2518301 | CCCTCTTCACTTCTCAGAATACATACGGTAAAATATACAAAAGAAGATTTTTCGACAA |
Chibazakura T, et al. (1991): S1 Eymann C, et al. (2001): NB |
| SigA | Promoter | -42:+12 | 2518253..2518306 | TTGATCCCTCTTCACTTCTCAGAATACATACGGTAAAATATACAAAAGAAGATT |
Predich M, et al. (1992): PE Eymann C, et al. (2001): NB |
| SigH | Promoter | -43:+4 | 2518102..2518148 | ATTTTTTTAGAGGGTATATAGCGGTTTTGTCGAATGTAAACATGTAG |
Predich M, et al. (1992): PE Chibazakura T, et al. (1995): S1 DB RG Eymann C, et al. (2001): NB DB; Western blot |
| Spo0A | Positive | -14:+1 | 2518110..2518123 | TTTGTCGAATGTAA |
Strauch MA, et al. (1992): FT |
| Spo0A | Negative | +38:+72 | 2518187..2518221 | AATTTCATTTTTAGTCGAAAAACAGAGAAAAACAT |
Strauch MA, et al. (1992): FT |
| Spo0A | Negative | -15:+20 | 2518239..2518263 | AAAAGAAGATTTTTCGACAAATTCA |
Strauch MA, et al. (1992): FT |
| Terminator sequence | Absolute position | Position from stop codon | Free energy [kcal/mol] |
Downstream of |
|---|---|---|---|---|
| GTCATTAAATCAAACGTCTTTTATTTATTAGTTTGCGCTGATAAATAGGAGGCGTTTTGTTTTGGGGAC >>>>>>>>>>>>>>>>>> <<<<<<<<<<<<<<<<<< |
spo0A |
|