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Copyright: Human Genome Center, Inst. Med. Sci., Univ. Tokyo; 1999-2006
Contact: Kenta Nakai |
| Regulated Operon: | spoVFAB-asd-dapG-dapA |
| Genes | Synonyms | Direction | Genome position | Function | COG ID | Genus clusters |
|---|---|---|---|---|---|---|
| spoVFA | dpaA | + | 1743659..1744552 | dipicolinate synthase subunit A | spoVFA-BAC | |
| spoVFB | dpaB | + | 1744555..1745157 | dipicolinate synthase subunit B | COG0452 | |
| asd | + | 1745283..1746323 | aspartate-semialdehyde dehydrogenase | COG0136 | asd-BAC asd-STA | |
| dapG | lssD | + | 1746415..1747629 | aspartokinase I (alpha and beta subunits) | COG0527 | |
| dapA | + | 1747660..1748532 | dihydrodipicolinate synthase | COG0329 | dapA-BAC x0966-BAC |
| Operon evidence: | primer extension analysis of dapG and dapA; integrative plasmids |
|---|---|
| Reference: | Chen NY, et al. (1993), Daniel RA & Errington J (1993) |
| Comments: | Internal promoter in front of asd. Second proposed terminator was found in a different section of the genome. |
| Binding factor |
Regulation | Location | Absolute position | Binding seq.(cis-element) | Experimental evidence |
|---|---|---|---|---|---|
| SigK | Promoter | -40:+11 | 1743566..1743616 | TTTTATATTCACCGGTATTTCCTTTTGATCATAAGATGAAGGGGAGCTTAA |
Chen NY, et al. (1993): PE Daniel RA & Errington J (1993): RG PE DB |
| SigA | Promoter | -41:+18 | 1745187..1745245 | AATTCGGTCATCACCCGCATATTCTATGGTAAAATAAAACGTAAAATCATAGTCAAATC |
Chen NY, et al. (1993): PE |
| unknown | Promoter | -46:+10 | 1745216..1745271 | TAAAATAAAACGTAAAATCATAGTCAAATCAATTCAATGAAGCTGATTGGCGGAAG |
Chen NY, et al. (1993): PE |
| Terminator sequence | Absolute position | Position from stop codon | Free energy [kcal/mol] |
Downstream of |
|---|---|---|---|---|
| AAACGCGGTCATTCTCACATTCAGCTGAGTTTGACCGTTTCTTTTACATATT >>>>>>>>>>>> <<<<<<<<<<<< |
dapA |
|