Regulated Operon: yqhV-spoIIIAABCDEFGH

Genes
Genes Synonyms Direction Genome position Function COG ID Genus clusters
yqhV yqgE - 2536921..2537202

yqgE-BAC  
spoIIIAA
- 2535922..2536845
COG3854  
spoIIIAB
- 2535413..2535928

 
spoIIIAC
- 2535184..2535390

spoIIIAC-BAC  
spoIIIAD
- 2534776..2535177

spoIIIAD-BAC  
spoIIIAE
- 2533540..2534757

 
spoIIIAF
- 2532923..2533543

 
spoIIIAG
- 2532241..2532930

 
spoIIIAH
- 2531584..2532240

 

Operon evidence: transcriptional fusions; Northern blotting (5.9 kb transcript)
Reference: Illing N & Errington J (1991), Yoshida K, et al. (2003), Genbank U35252
Comments: Transcriptional fusions showed that spoIIIA contains at least the A, B, and C genes. Northern blotting revealed a yqhV-spoIIIAABCDEFGH transcript. The promoter in front of spoIIIAA may therefore be an internal promoter. BSORF lists a spoIIIAABCDEFGH transcript, in addition to shorter spoIIIAGH and spoIIIAH transcripts.

Promoters

Binding
factor		 Regulation Location Absolute position Binding seq.(cis-element) Experimental evidence
YlbO Negative ND ND ND Eichenberger P, et al. (2004): AR DB RG
SigE Promoter -39:+3 2536872..2536913 CTTGTCATAAAGTCTGCCTCACATCATACATTTTAAAGAAGC Illing N & Errington J (1991): RG DB PE OV
SpoIIID Negative -54:+1 2536875..2536929 CATAAATAAAGAGAGGCTTGTCATAAAGTCTGCCTCACATCATACATTTTAAAGA Eichenberger P, et al. (2004): FT AR
SigE Promoter -40:+14 2533240..2533293 AAATAGAAATACAAGCTTCCCAGCGCGCATATATTCTAGAAGAAATGGCTGTCC Stragier P (2003): personal communication: PE RG

Terminator

Terminator sequence Absolute position Position from stop codon Free energy
[kcal/mol]		 Downstream of
AAAAAGCCCGCTAAACAAGCGGGCTTTTTGCGTTGCTGT
     >>>>>>>     <<<<<<<
2531550..2531568
16..34
-15.5
 spoIIIAH

Overview



Upper Region
Copyright: Human Genome Center, Inst. Med. Sci., Univ. Tokyo; 1999-2006
Contact: Kenta Nakai