Regulated Operon: yqxD-dnaG-sigA

Genes
Genes Synonyms Direction Genome position Function COG ID Genus clusters
yqxD yqfM - 2602606..2603196
COG1671  
dnaG dnaE - 2600761..2602572 DNA primase COG0358 dnaG-OYP-1 dnaG-OYP-2 dnaG-STA dnaG-STR dnaE-STA dnaE-STR  
sigA rpoD, crsA - 2599447..2600562 RNA polymerase major sigma-43 factor (sigma-A) COG0568 sigA-STA rpoD-OYP-2 sigA-STA  

Operon evidence: subcloning into a terminator-probe plasmid
Reference: Wang LF & Doi RH (1986), Wang LF & Doi RH (1987)
Comments: Internal promoters in front of dnaG and sigA. For the dnaG-sigA transcript, dnaG is transcribed but not translated.

Promoters

Binding
factor		 Regulation Location Absolute position Binding seq.(cis-element) Experimental evidence
SigA Promoter -42:+5 2603255..2603301 AACAATAGCATCTTTGTGAAGTTTGTATTATAATAAAAAATTGTGAT Wang LF, et al. (1987): S1
SigA Promoter -42:+4 2603283..2603328 TGGCTGTGCCAAAAGGGAATAATGAAAAACAATAGCATCTTTGTGA Wang LF, et al. (1987): S1
SigB Promoter -41:+8 2603292..2603340 ATCCTGGGTTTTTGGCTGTGCCAAAAGGGAATAATGAAAAACAATAGCA Liao CT, et al. (1999): PE
unknown Promoter -43:+4 2603200..2603246 TAATTTTAGGTTTAAGGATCGTGTGATACGAATAAACTATTATGGGT Qi FX, et al. (1991): PE DB
SigH Promoter -42:+7 2602579..2602627 CGATGAAGGGATTTTGCACTAAAGCATCGAATAATGTACGACGGAGTGT Qi FX & Doi RH (1990): PE RG
SigH Promoter -46:+5 2603069..2603119 GCTTTTATGCAGGAGTTTAATGGAGGGATGGAGAATTACTCTTCTTAATGA Carter HL 3rd, et al. (1988): PE DB RG
SigD Promoter ND 2600755..2600807 CTTCTTTGGCTCAAGAAATCGTTACATTGAACCGATCTTTAAAATAACTGGAG Wang LF, et al. (1985): HM
Briat JF, et al. (1985): S1

Terminator

Terminator sequence Absolute position Position from stop codon Free energy
[kcal/mol]		 Downstream of
AAGATGGAACGGGTCTTGAAGATCCGTTCTTCTTTTTTTAAAAAGAT
     >>>>>>>>>>>   <<<<<<<<<<<
2599419..2599443
4..28
-18.4
 sigA

Overview



Upper Region
Copyright: Human Genome Center, Inst. Med. Sci., Univ. Tokyo; 1999-2006
Contact: Kenta Nakai