Regulated Operon: levDEFG-sacC

Genes
Genes Synonyms Direction Genome position Function COG ID Conserved groups
levD sacL - 2762395..2762835
COG2893G  
levE sacL - 2761907..2762395
COG3444G  
levF sacL - 2761081..2761890
COG3715G  
levG sacL - 2760233..2761060
COG3716G  
sacC sacL - 2758043..2760076
COG1621G  

Operon evidence: 5' primer extension mapping of the sacC gene
Reference: Martin I, et al. (1989), Martin I, et al. (1987)
Comments:


Promoters

Binding
factor
Regulation Location Absolute position Binding seq.(cis-element) Experimental evidence
CcpA Negative -50:-36 2762900..2762914 TGAAAACGCTTAACA Martin-Verstraete I, et al. (1995): SDM DB
LevR Positive -148:-106 2762968..2763010 TATGAACCTGTATTAAATGGAACACCATTTTAATACAGGTTTA Debarbouille M, et al. (1991): DB RG
Martin-Verstraete I, et al. (1994): DP DB GS FT
LevR Positive -92:-69 2762931..2762954 AAGTGTTTCAACAACAAATTGCTA Debarbouille M, et al. (1991): DB RG
Martin-Verstraete I, et al. (1994): DP SDM GS FT
SigL Promoter -35:+5 2762858..2762897 ACTGTGTTGGCACGATCCTTGCATTATATATGGATGTACA Martin I, et al. (1989): PE
Debarbouille M, et al. (1991): DB RG

Terminator

Terminator sequence Absolute position Position from stop codon Free energy
[kcal/mol]
Downstream of
AAAAAGGCACAGACCCGAACAGTCTGTGCCTTCTTGTTATTTTAA
     >>>>>>>>>       <<<<<<<<<
2758008..2758032
11..35
-17.4
sacC

Overview



Upper Region






Copyright: Human Genome Center, Inst. Med. Sci., Univ. Tokyo; 1999-2008
Contact: Kenta Nakai