Regulated Operon: murE-mraY-murD-spoVE-murG-murB-divIB-ylxWX-sbp

Genes
Genes Synonyms Direction Genome position Function COG ID Conserved groups
murE
+ 1586330..1587814
COG0769M murE-BAC murE-STA murE-STR  
mraY
+ 1587926..1588900
COG0472M  
murD
+ 1588901..1590256
COG0771M murD-CLO murD-STR  
spoVE
+ 1590317..1591417
COG0772D spoVE-BAC  
murG
+ 1591540..1592631
COG0707M murG-BAC murG-STA  
murB ylxC + 1592663..1593574
COG0812M murB-STR x1153-STA  
divIB tms-12 + 1593704..1594495
COG1589M  
ylxW
+ 1594492..1595187
COG3879S  
ylxX
+ 1595210..1595917
COG3879S  
sbp
+ 1595935..1596300
COG3856S sbp-CLO  

Operon evidence: Northern blotting (transcripts of 8.3, 6.0, 5.9, 4.9, 1.2, and 0.85 kb)
Reference: Henriques AO, et al. (1992), Harry EJ, et al. (1994)
Comments: The stem-loop structure downstream of spoVE may function as a read-through terminator. Northern blotting results suggest that the stem-loop structure downstream of murB does not function as a transcriptional terminator.

Promoters

Binding
factor		 Regulation Location Absolute position Binding seq.(cis-element) Experimental evidence
SigE Promoter -37:+6 1590218..1590260 GGTGACATGTTTATAGATGCCGTGCATATGCTTAAGTAAGGGC Theeragool G, et al. (1993): PE S1 RG DB
Miyao A, et al. (1993): RO, RNAP purification
SigE Promoter -40:+9 1590077..1590125 CTGGGAATACAACATGTCAAACGTGTCGATAATGTTGAACAAGCAGTAT Theeragool G, et al. (1993): PE S1 RG DB
Miyao A, et al. (1993): RO, RNAP purification
SpoIIID Negative -38:+21 1590079..1590137 GGGAATACAACATGTCAAACGTGTCGATAATGTTGAACAAGCAGTATCTGCGGCGTTTG Eichenberger P, et al. (2004): FT AR
SpoIIID Negative -38:+21 1590218..1590276 GGTGACATGTTTATAGATGCCGTGCATATGCTTAAGTAAGGGCTTGTCTTGAAGTAAAT Eichenberger P, et al. (2004): FT AR
SigA Promoter -50:+15 1593567..1593631 ATCGCTGATTCAAGTTCTGACTGAAGCTGTTCATATGATATACTGTAAGCAAACGACAAACGGCA Harry EJ, et al. (1994): PE DB SDM RG DP NB

Terminator

Terminator sequence Absolute position Position from stop codon Free energy
[kcal/mol]		 Downstream of
GGAAACAATTGATGCGATAACCCTGTCCAACAAAACAGGGTTATCGTTATGTTATAGAGAA
              >>>>>>>>>>>>        <<<<<<<<<<<<
1591470..1591501
53..84
-20.0
 spoVE

Overview



Upper Region
Copyright: Human Genome Center, Inst. Med. Sci., Univ. Tokyo; 1999-2008
Contact: Kenta Nakai