Regulated Operon: nfrA-ywcH

Genes
Genes Synonyms Direction Genome position Function COG ID Conserved groups
nfrA ipa-43d - 3911477..3912226
COG0778C  
ywcH ipa-44d - 3910302..3911303
COG2141C x0289-BAC-1  

Operon evidence: Northern blotting (2.0 kb transcript for nfrA-ywcH, 0.7 kb transcript for nfrA)
Reference: Moch C, et al. (2000), Nguyen TT, et al. (2009)
Comments: The stem-loop between nfrA and ywcH may function as a readthrough terminator, although the Northern blotting experiment indicates a nfrA-ywcH operon.

Promoters

Binding
factor		 Regulation Location Absolute position Binding seq.(cis-element) Experimental evidence
SigA Promoter -41:+4 3912242..3912286 TTTCACTTTTGAGATCACTTTTTTTCGGATATGATAAAAAGTGAT Moch C, et al. (2000): PE
SigD Promoter -41:+5 3912241..3912286 TTTCACTTTTGAGATCACTTTTTTTCGGATATGATAAAAAGTGATA Moch C, et al. (1998): PE SDM HM
Spo0A Negative ND ND ND Molle V, et al. (2003): GS CH
Spo0A Negative ND ND ND Molle V, et al. (2003): GS CH

Terminator

Terminator sequence Absolute position Position from stop codon Free energy
[kcal/mol]		 Downstream of
AAAAAGCCCTGCCGAATCGGCAGGGCTTTTTCTTATTCAAC
     >>>>>>>>>   <<<<<<<<<
3910271..3910291
11..31
-20.0
 ywcH
AAAGCAAACCTCTCCGTCTGGAGGGGTTTTTATGTTTCACGTGTAAC
     >>>>>>>>>>    <<<<<<<<<<
3911450..3911473
4..27
-13.1
 nfrA

Overview



Upper Region
Copyright: Human Genome Center, Inst. Med. Sci., Univ. Tokyo; 1999-2008
Contact: Kenta Nakai