Regulated Operon: skf

Genes
Genes Synonyms Direction Genome position Function COG ID Conserved groups
ybcO skfA + 213926..214093

 
ybcP ybcQ, skfB, ybcQ + 214160..215392

 
ybcS skfC + 215389..215946

 
ybcT skfD + 215943..216878

 
ybdA skfE + 216897..217616

 
ybdB skfF + 217681..219027

 
ybdD skfG + 219074..219487

 
ybdE skfH + 219593..220018

 

Operon evidence: Northern blotting (6.5 kb transcript)
Reference: Gonzalez-Pastor JE, et al. (2003), Allenby NEE, et al. (2006)
Comments: Northern blotting during during phosphate limitation showed an intense 0.25 kb skfA-specific transcript, and a weaker 6.5 kb skfABCDEFGh transcript. The skfA transcript is stabilized by a stem-loop structure in the skfA-skfB intergenic region.

Promoters

Binding
factor		 Regulation Location Absolute position Binding seq.(cis-element) Experimental evidence
AbrB Negative ND ND ND Fujita M, et al. (2005): RG
PhoP Positive ND ND ND Allenby NEE, et al. (2006): DB RG NB GS
SigA Promoter -64:+4 213824..213891 AATTTTTAGGATAATATACAAAATCCCCCTTACTTCGACAATTGCAATCTGGTATTATCGTATCGCAT Chen G, et al. (2006): PE
Spo0A Positive -64:+4 213824..213891 AATTTTTAGGATAATATACAAAATCCCCCTTACTTCGACAATTGCAATCTGGTATTATCGTATCGCAT Molle V, et al. (2003): GS CH
Fujita M, et al. (2005): RG OV, gfp fusion, GS
Chen G, et al. (2006): FT, binding site mutation

Terminator

Terminator sequence Absolute position Position from stop codon Free energy
[kcal/mol]		 Downstream of
TAACATTTGAGAATAGGGAGTTGAGCGTATTTGCTTATACTCCTTATTTTCTCTTAAG
                >>>>>>>>>>      <<<<<<<<<<<
214122..214148
29..55
-13.4
 ybcO
ATGAAACGGTCCTTCCTATAAGCAGGAAGGTTTTTTAATGAAATA
          >>>>>>>      <<<<<<<
220085..220104
67..86
-10.9
 ybdE

Overview



Upper Region
Copyright: Human Genome Center, Inst. Med. Sci., Univ. Tokyo; 1999-2008
Contact: Kenta Nakai