Regulated Operon: spoIVCB-spoIIIC

Genes
Genes Synonyms Direction Genome position Function COG ID Conserved groups
spoIVCB cisB, sigK, sigK + 2652219..2652689 RNA polymerase sporulation-specific sigma factor (sigma-K) (N-terminal half)
sigK-BAC sigK-BAC  
spoIIIC sigK, spoIVD, spoIVE, spoIVE + 2700564..2700980 RNA polymerase sporulation-specific sigma factor (sigma-K) (C-terminal half)
sigK-BAC sigK-BAC  

Operon evidence: upstream and downstream genes are in opposite directions
Reference: Stragier P, et al. (1989), Errington J, et al. (1988)
Comments: spoIVCB and spoIIIC form a composite gene in the mother cell during sporulation by excising the intervening DNA sequence.

Promoters

Binding
factor		 Regulation Location Absolute position Binding seq.(cis-element) Experimental evidence
GerE Negative -9:+22 2652945..2652975 ATACATTTACATATAGGCTTTTGCCTACATA Zheng L, et al. (1992): RO
Ichikawa H, et al. (1999): FT RG
GerE Negative -1:+31 2652953..2652984 ACATATAGGCTTTTGCCTACATACTTTTGTGG Zheng L, et al. (1992): RO
Ichikawa H, et al. (1999): FT RG
SigE Promoter -39:+9 2652915..2652962 CGGTACAGACACAGACAGCCTCCCGGTCACATACATTTACATATAGGC Kunkel B, et al. (1988): PE RG
SigK Promoter -39:+9 2652915..2652962 CGGTACAGACACAGACAGCCTCCCGGTCACATACATTTACATATAGGC Kunkel B, et al. (1988): PE RG
Kroos L, et al. (1989): RO, SDS-PAGE
SpoIIID Positive -36:-16 2652918..2652938 TACAGACACAGACAGCCTCCC Halberg R, et al. (1994): FT RO
SpoIIID Positive +78:+100 2653031..2653053 TAAAGAGCTTGTCTTTTTAGTAT Halberg R, et al. (1994): FT RO
SpoIIID Positive +109:+133 2653062..2653086 AAAAACAATGCCTTTCCACAACCGC Halberg R, et al. (1994): FT RO

Terminator

Terminator sequence Absolute position Position from stop codon Free energy
[kcal/mol]		 Downstream of
AAGAAGCCGGATCTCATACTCCGGCTTTCTCTATTTGAAA
     >>>>>>        <<<<<<
2701765..2701784
785..804
-11.6
 spoIIIC

Overview



Upper Region
Copyright: Human Genome Center, Inst. Med. Sci., Univ. Tokyo; 1999-2008
Contact: Kenta Nakai