Regulated Operon: araABDLMNPQ-abfA

Genes
Genes Synonyms Direction Genome position Function COG ID Genus clusters
araA
- 2946453..2947949 L-arabinose isomerase COG2160  
araB
- 2944757..2946439 L-ribulokinase COG1069  
araD
- 2944051..2944740 L-ribulose-5-phosphate 4-epimerase COG0235 araD-BAC  
araL yseA - 2943255..2944073
COG0647  
araM yseB - 2942074..2943258
COG0371  
araN yseC - 2940742..2942043 sugar-binding protein COG1653  
araP yseD - 2939765..2940706 integral membrane protein COG1175  
araQ yseE - 2938919..2939764 integral membrane protein COG0395  
abfA
- 2937398..2938900 alpha-L-arabinofuranosidase COG3534  

Operon evidence: Northern blotting (11.0 kb transcript)
Reference: Sa Nogueira I & Ramos SS (1997), Genbank X89810
Comments: premature transcription termination or internal promoters may have lead to other detected mRNA lengths. One more putative terminator is listed in the Genbank record.

Promoters

Binding
factor		 Regulation Location Absolute position Binding seq.(cis-element) Experimental evidence
AraR Negative -7:+17 2948032..2948055 TAAAATTGTTCGTACAAAATATTT Sa Nogueira & Mota LJ (1997): DB HM
Mota LJ, et al. (1999): FT
AraR Negative +38:+58 2947989..2948009 CATTAGTACGTATCTTTTGTA Sa Nogueira I & Mota LJ (1997): DB HM
Mota LJ, et al. (1999): FT
CcpA Negative +50:+83 2947963..2947996 CTTTTGTATTTGAAAGCGTTTTATTTTATGAGAA Inacio JM, et al. (2003): RG NB
SigA Promoter -40:+6 2948043..2948088 AAAGATTGACAGTATAATAGTCAATTACTATAATAAAATTGTTCGT Sa Nogueira I & Ramos SS (1997): PE HM
CcpA Negative ND 2945742..2945764 TCAATGAAAACGATTACAAAGGA Miwa Y, et al. (2000): RG

Terminator

Terminator sequence Absolute position Position from stop codon Free energy
[kcal/mol]		 Downstream of
GCAAAGCCGGAGATTTCTCTCCGGCTTGTCTTTCAACTGC
     >>>>>>>>    <<<<<<<<
2937368..2937387
11..30
-16.4
 abfA

Overview



Upper Region
Copyright: Human Genome Center, Inst. Med. Sci., Univ. Tokyo; 1999-2006
Contact: Kenta Nakai