Regulated Operon: citZ-icd-mdh

Genes
Genes Synonyms Direction Genome position Function COG ID Genus clusters
citZ citA2 - 2980190..2981308 citrate synthase II COG0372 citZ-BAC citZ-STA  
icd citC - 2978755..2980026 isocitrate dehydrogenase COG0538 citC-BAC citC-STA citC-BAC citC-STA  
mdh citH - 2977773..2978711 malate dehydrogenase COG0039 mdh-BAC-1 mdh-BAC-2 citM-BAC mdh-BAC-2  

Operon evidence: Genome analysis
Reference: Jin S & Sonenshein AL (1994), Jin S, et al. (1996), Lapidus A, et al. (1997), Genbank AF008220
Comments: internal promoters in front of icd and mdh


Promoters

Binding
factor
Regulation Location Absolute position Binding seq.(cis-element) Experimental evidence
CcpA Negative +80:+97 2981406..2981423 AATGTAAGCATTTTCTTT Kim HJ, et al. (2002): PE DB RG SDM GS
CcpC Negative -11:+5 2981498..2981513 ATAATGAGAATAGGCT Jourlin-Castelli C, et al. (2000): RG GS FT
Kim HJ, et al. (2002): PE DB RG SDM FT
CcpC Negative +21:+44 2981459..2981482 TTATAAAAATTTGTTATGTTCTTT Jourlin-Castelli C, et al. (2000): RG GS FT
Kim HJ, et al. (2002): PE DB RG SDM FT
SigA Promoter -48:+18 2981485..2981550 TATAGGTAAACATTTAACAAATGTCTGATTATTGTTTATAATGAGAATAGGCTTAAACTTAAATAA Jin S, et al. (1994): PE
SigA Promoter -45:+18 2980091..2980153 GCCTCTTGTTCAGACATCAAAATTGGGTTACACTTTAAATTGAATGTTAGGAAAAATCATTTT Jin S, et al. (1994): PE

Terminator

Terminator sequence Absolute position Position from stop codon Free energy
[kcal/mol]
Downstream of
AGAAAGGCTTGCTTAATACAGCCTTTCTCTTTTTACTA
     >>>>          <<<<
2977741..2977758
15..32
-4.8
mdh

Overview



Upper Region






Copyright: Human Genome Center, Inst. Med. Sci., Univ. Tokyo; 1999-2006
Contact: Kenta Nakai