Regulated Operon: mmsA-iolBCDEF-idh-iolHI-fbaB

Genes
Genes Synonyms Direction Genome position Function COG ID Genus clusters
mmsA iolA, yxdA - 4081939..4083402 methylmalonate-semialdehyde dehydrogenase COG1012 x1017-BAC  
iolB yxdB - 4081049..4081864
COG3718  
iolC yxdC - 4080048..4081025
COG0524 iolC-BAC-1 iolC-BAC-2  
iolD yxdD - 4078102..4079844
COG3962  
iolE yxdE - 4077192..4078085
COG1082 iolE-BAC  
iolF yxdF - 4075858..4077177 inositol transport protein COG0477  
idh iolG, iol - 4074801..4075835 myo-inositol 2-dehydrogenase COG0673 idh-BAC idh-BAC  
iolH yxdG - 4073912..4074781
COG1082  
iolI yxdH - 4072990..4073826
COG1082  
fbaB iolJ, yxdI - 4072097..4072969 fructose-1,6-bisphosphate aldolase COG0191 fbaA-BAC  

Operon evidence: S1 nuclease mapping of the 3' end; Northern blotting (11.5 kb transcript)
Reference: Yoshida K, et al. (1994), Yoshida KI, et al. (1997), Yoshida K, et al. (2000)
Comments: The proposed terminator agrees well with the experimentally determined termination site in the TTTTTTT stretch.

Promoters

Binding
factor		 Regulation Location Absolute position Binding seq.(cis-element) Experimental evidence
CcpA Negative +83:+110 4083484..4083511 TTTTTGAAAGCGTTTAATTCTTGGCTTG Miwa Y, et al. (2000): DP SDM
IolR Negative -107:-45 4083638..4083700 CCTTCTCTTACTTCTCTTACTTGATTAAAAGATTAATATAATAAAAATAATGAAAAAATGTAG Yoshida KI, et al. (1997): DB DP HB
IolR Negative -5:+17 4083577..4083598 TAACCAAGAAATGACCAAAAAG Yoshida KI, et al. (1999): GS FT
SigA Promoter -39:+8 4083586..4083632 ATGATTGACTTATGGGTATTATGCGATTAGAATATAACCAAGAAATG Yoshida KI, et al. (1997): PE
CcpA Negative ND 4081178..4081200 GAAATGAAAACGTTGTCATCGTT Miwa Y, et al. (2000): RG

Terminator

Terminator sequence Absolute position Position from stop codon Free energy
[kcal/mol]		 Downstream of
GAAACCGCCGAGATGGCGTGTTTTTTTGTGCGGTG
     >>>>     <<<<
4072071..4072083
14..26
-8.1
 fbaB

Overview



Upper Region
Copyright: Human Genome Center, Inst. Med. Sci., Univ. Tokyo; 1999-2006
Contact: Kenta Nakai