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Copyright: Human Genome Center, Inst. Med. Sci., Univ. Tokyo; 1999-2006
Contact: Kenta Nakai |
| Regulated Operon: | murE-mraY-murD-spoVE-murG-murB-divIB-ylxWX-sbp |
| Genes | Synonyms | Direction | Genome position | Function | COG ID | Genus clusters |
|---|---|---|---|---|---|---|
| murE | + | 1585631..1587115 | UDP-N-acetylmuramoylalanyl-D-glutamate-2,6- diaminopimelate ligase | COG0769 | murE-BAC murE-STA murE-STR | |
| mraY | + | 1587228..1588202 | phospho-N-acetylmuramoyl-pentapeptide transferase | COG0472 | ||
| murD | + | 1588203..1589558 | UDP-N-acetylmuramoylalanyl-D-glutamate ligase | COG0771 | murD-CLO murD-STR | |
| spoVE | + | 1589619..1590719 | COG0772 | spoVE-BAC | ||
| murG | + | 1590842..1591933 | UDP-N-acetylglucosamine-N-acetylmuramyl- (pentapeptide)pyrophosphoryl-undecaprenol N-acetylglucosamine transferase | COG0707 | murG-BAC murG-STA | |
| murB | ylxC | + | 1591960..1592871 | UDP-N-acetylenolpyruvoylglucosamine reductase | COG0812 | murB-STR x1153-STA |
| divIB | dds, tms-12 | + | 1593002..1593793 | cell-division initiation protein | COG1589 | |
| ylxW | + | 1593790..1594485 | COG3879 | |||
| ylxX | + | 1594508..1595215 | COG3879 | |||
| sbp | + | 1595233..1595598 | small basic protein | COG3856 | sbp-CLO |
| Operon evidence: | Northern blotting (transcripts of 8.3, 6.0, 5.9, 4.9, 1.2, and 0.85 kb) |
|---|---|
| Reference: | Henriques AO, et al. (1992), Harry EJ, et al. (1994) |
| Comments: | The stem-loop structure downstream of spoVE may function as a read-through terminator. Northern blotting results suggest that the stem-loop structure downstream of murB does not function as a transcriptional terminator. |
| Binding factor |
Regulation | Location | Absolute position | Binding seq.(cis-element) | Experimental evidence |
|---|---|---|---|---|---|
| SigE | Promoter | -37:+6 | 1589520..1589562 | GGTGACATGTTTATAGATGCCGTGCATATGCTTAAGTAAGGGC |
Theeragool G, et al. (1993): PE S1 RG DB Miyao A, et al. (1993): RO, RNAP purification |
| SigE | Promoter | -40:+9 | 1589379..1589427 | CTGGGAATACAACATGTCAAACGTGTCGATAATGTTGAACAAGCAGTAT |
Theeragool G, et al. (1993): PE S1 RG DB Miyao A, et al. (1993): RO, RNAP purification |
| SpoIIID | Negative | -38:+21 | 1589381..1589439 | GGGAATACAACATGTCAAACGTGTCGATAATGTTGAACAAGCAGTATCTGCGGCGTTTG |
Eichenberger P, et al. (2004): FT AR |
| SpoIIID | Negative | -38:+21 | 1589520..1589578 | GGTGACATGTTTATAGATGCCGTGCATATGCTTAAGTAAGGGCTTGTCTTGAAGTAAAT |
Eichenberger P, et al. (2004): FT AR |
| SigA | Promoter | -45:+19 | 1592869..1592932 | TGATTCAAGTTCTGACTGAAGCTGTTCATATGACATACTGTAAGCAAACGAACAAACGGCATCA |
Harry EJ, et al. (1994): PE DB SDM RG DP NB |
| Terminator sequence | Absolute position | Position from stop codon | Free energy [kcal/mol] |
Downstream of |
|---|---|---|---|---|
| GGAAACAATTGATGCGATAACCCTGTCCAACAAAACAGGGTTATCGTTATGTTATAGAGAA >>>>>>>>>>>> <<<<<<<<<<<< |
spoVE |
|