Regulated Operon: murE-mraY-murD-spoVE-murG-murB-divIB-ylxWX-sbp

Genes
Genes Synonyms Direction Genome position Function COG ID Genus clusters
murE
+ 1585631..1587115 UDP-N-acetylmuramoylalanyl-D-glutamate-2,6- diaminopimelate ligase COG0769 murE-BAC murE-STA murE-STR  
mraY
+ 1587228..1588202 phospho-N-acetylmuramoyl-pentapeptide transferase COG0472  
murD
+ 1588203..1589558 UDP-N-acetylmuramoylalanyl-D-glutamate ligase COG0771 murD-CLO murD-STR  
spoVE
+ 1589619..1590719
COG0772 spoVE-BAC  
murG
+ 1590842..1591933 UDP-N-acetylglucosamine-N-acetylmuramyl- (pentapeptide)pyrophosphoryl-undecaprenol N-acetylglucosamine transferase COG0707 murG-BAC murG-STA  
murB ylxC + 1591960..1592871 UDP-N-acetylenolpyruvoylglucosamine reductase COG0812 murB-STR x1153-STA  
divIB dds, tms-12 + 1593002..1593793 cell-division initiation protein COG1589  
ylxW
+ 1593790..1594485
COG3879  
ylxX
+ 1594508..1595215
COG3879  
sbp
+ 1595233..1595598 small basic protein COG3856 sbp-CLO  

Operon evidence: Northern blotting (transcripts of 8.3, 6.0, 5.9, 4.9, 1.2, and 0.85 kb)
Reference: Henriques AO, et al. (1992), Harry EJ, et al. (1994)
Comments: The stem-loop structure downstream of spoVE may function as a read-through terminator. Northern blotting results suggest that the stem-loop structure downstream of murB does not function as a transcriptional terminator.


Promoters

Binding
factor
Regulation Location Absolute position Binding seq.(cis-element) Experimental evidence
SigE Promoter -37:+6 1589520..1589562 GGTGACATGTTTATAGATGCCGTGCATATGCTTAAGTAAGGGC Theeragool G, et al. (1993): PE S1 RG DB
Miyao A, et al. (1993): RO, RNAP purification
SigE Promoter -40:+9 1589379..1589427 CTGGGAATACAACATGTCAAACGTGTCGATAATGTTGAACAAGCAGTAT Theeragool G, et al. (1993): PE S1 RG DB
Miyao A, et al. (1993): RO, RNAP purification
SpoIIID Negative -38:+21 1589381..1589439 GGGAATACAACATGTCAAACGTGTCGATAATGTTGAACAAGCAGTATCTGCGGCGTTTG Eichenberger P, et al. (2004): FT AR
SpoIIID Negative -38:+21 1589520..1589578 GGTGACATGTTTATAGATGCCGTGCATATGCTTAAGTAAGGGCTTGTCTTGAAGTAAAT Eichenberger P, et al. (2004): FT AR
SigA Promoter -45:+19 1592869..1592932 TGATTCAAGTTCTGACTGAAGCTGTTCATATGACATACTGTAAGCAAACGAACAAACGGCATCA Harry EJ, et al. (1994): PE DB SDM RG DP NB

Terminator

Terminator sequence Absolute position Position from stop codon Free energy
[kcal/mol]
Downstream of
GGAAACAATTGATGCGATAACCCTGTCCAACAAAACAGGGTTATCGTTATGTTATAGAGAA
              >>>>>>>>>>>>        <<<<<<<<<<<<
1590772..1590803
53..84
-20.0
spoVE

Overview



Upper Region






Copyright: Human Genome Center, Inst. Med. Sci., Univ. Tokyo; 1999-2006
Contact: Kenta Nakai